Inducibility of Metallothionein mRNA Expression and Cadmium Tolerance in Larvae of a Marine Teleost, the Turbot (Scophthalmus maximus)
An acute (48-hr) lethal toxicity study of turbot at various stages from hatch to juveniles showed that prior to first exogenous feed-ing, larvae of turbot were an order of magnitude more sensitive to cadmium toxicity than later developmental stages (L.C50 of 0.18-0.23 ppm Cd versus 2-5 ppm Cd). To i...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Text |
| Language: | English |
| Published: |
1995
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| Subjects: | |
| Online Access: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.505.834 http://toxsci.oxfordjournals.org/content/33/1/91.full.pdf |
| Summary: | An acute (48-hr) lethal toxicity study of turbot at various stages from hatch to juveniles showed that prior to first exogenous feed-ing, larvae of turbot were an order of magnitude more sensitive to cadmium toxicity than later developmental stages (L.C50 of 0.18-0.23 ppm Cd versus 2-5 ppm Cd). To investigate the possible role of metallothionein (MT) in Cd tolerance, the concentrations of MT mRNA were determined in control and Cd-exposed animals using a plaice MT cDNA probe. MT mRNA was expressed throughout embryonic and early larval development at levels ap-proximately twice those in juvenile turbot liver apart from the period around hatching, when there was a decreased level of ex-pression. During endogenous feeding, despite larval drinking, MT mRNA levels did not appear to be inducible by exposure to Cd in the water within 3 days of hatching but were induced three- to fivefold after 48 hr exposure to waterborne Cd at all later stages |
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