Summary
Fusel oil is a by-product of distilleries, its main component is i-amyl alcohol, which can form ester compounds. Esterification of oleic acid and i-amyl alcohol by Candida antarctica lipase B (Novozym 435 preparation) in n-heptane solvent was studied in this work. Ping--pong bi-bi mechanism (inhibit...
Main Authors: | , , , , |
---|---|
Other Authors: | |
Format: | Text |
Language: | English |
Published: |
2006
|
Subjects: | |
Online Access: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.455.1811 http://www.ftb.pbf.hr/import/46-1-6.pdf |
Summary: | Fusel oil is a by-product of distilleries, its main component is i-amyl alcohol, which can form ester compounds. Esterification of oleic acid and i-amyl alcohol by Candida antarctica lipase B (Novozym 435 preparation) in n-heptane solvent was studied in this work. Ping--pong bi-bi mechanism (inhibition phenomena taken into account) was applied as a com-plex kinetic model. The parameters of the model were determined by numerical methods. It was found that four-parameter model fitted well with the experimental results and de-scribed properly the enzymatic reaction. Key words: fusel oil, esterification, lipase, ping-pong bi-bi mechanism |
---|