Mitochondrial DNA Downloaded from informahealthcare.com by Dr. Sergios-Orestis Kolokotronis

DNA barcoding efforts involving animals have focused on the mitochondrial cytochrome c oxidase subunit I (Cox1) gene. Some authors suggest that this marker might under-diagnose young species. Herein, we examine Cox1 and control region diversity in a sample of Siberian sturgeon (Acipenser baerii), a...

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Bibliographic Details
Main Authors: Vadim J. Birstein, Rob Desalle, Phaedra Doukakis, Robert Hanner, Georgii I. Ruban, Eugene Wong
Other Authors: The Pennsylvania State University CiteSeerX Archives
Format: Text
Language:English
Published: 2009
Subjects:
DNA barcode
Cox1
control region
forensics
sturgeon
subspecies
Acipenser baerii
Siberian sturgeon
Siberia
Online Access:http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.419.4299
http://research.amnh.org/users/desalle/pdf/Birstein.2009.MTDNA.sturgeon.pdf
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Summary:DNA barcoding efforts involving animals have focused on the mitochondrial cytochrome c oxidase subunit I (Cox1) gene. Some authors suggest that this marker might under-diagnose young species. Herein, we examine Cox1 and control region diversity in a sample of Siberian sturgeon (Acipenser baerii), a species with an extremely wide geographic distribution in the major rivers of Siberia and in Lake Baikal. Some authors currently recognize three subspecies within this species. These subspecies are reasonable candidates for species units detectable through DNA barcoding. The Cox1 gene illustrated no variation within the species, while the control region displayed statistically significant differences among the subspecies using analysis of molecular variance (AMOVA). Given the uniformity of Cox1 sequences recovered, Cox1 is probably a good region for barcoding A. baerii at the species level. Although control region variation among subspecies was significant, diagnostic differences were not found for any of the subspecies.

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