www.mdpi.com/journal/ijms High Yield of Wax Ester Synthesized from Cetyl Alcohol and Octanoic Acid by Lipozyme RMIM and Novozym 435

Abstract: Wax esters are long-chain esters that have been widely applied in premium lubricants, parting agents, antifoaming agents and cosmetics. In this study, the biocatalytic preparation of a specific wax ester, cetyl octanoate, is performed in n-hexane using two commercial immobilized lipases, i...

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Bibliographic Details
Main Authors: Chia-hung Kuo, Hsin-hung Chen, Jiann-hwa Chen, Yung-chuan Liu, Chwen-jen Shieh
Other Authors: The Pennsylvania State University CiteSeerX Archives
Format: Text
Language:English
Published: 2012
Subjects:
optimization
wax esters
lipase
esterification
Rhizomucor miehei
Candida antarctica
Antarc*
Antarctica
Online Access:http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.383.63
http://www.mdpi.com/1422-0067/13/9/11694/pdf/
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Summary:Abstract: Wax esters are long-chain esters that have been widely applied in premium lubricants, parting agents, antifoaming agents and cosmetics. In this study, the biocatalytic preparation of a specific wax ester, cetyl octanoate, is performed in n-hexane using two commercial immobilized lipases, i.e., Lipozyme ® RMIM (Rhizomucor miehei) and Novozym ® 435 (Candida antarctica). Response surface methodology (RSM) and 5-level-4-factor central composite rotatable design (CCRD) are employed to evaluate the effects of reaction time (1–5 h), reaction temperature (45–65 °C), substrate molar ratio (1–3:1), and enzyme amount (10%–50%) on the yield of cetyl octanoate. Using RSM to optimize the reaction, the maximum yields reached 94 % and 98 % using Lipozyme ® RMIM and Novozym ® 435, respectively. The optimum conditions for synthesis of cetyl octanoate by both lipases are established and compared. Novozym ® 435 proves to be a more efficient

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