pipistrellus (Vespertilionidae; Chiroptera)

This paper reports the molecular and cytogenetic characterization of a HindIII family of satellite DNA in the bat species Pipistrellus pipistrellus. This satellite is organized in tandem repeats of 418 bp monomer units, and represents approximately 3 % of the whole genome. The consensus sequence fro...

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Bibliographic Details
Main Authors: Mjl Barragán, S Martínez, Ja Marchal, R Fernández, M Bullejos, Rdíaz De La Guardia
Other Authors: The Pennsylvania State University CiteSeerX Archives
Format: Text
Language:English
Subjects:
Pipistrellus pipistrellus
microchiroptera
repetitive DNA
pericentric satellite DNA
The ''Y''
Online Access:http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.378.9001
http://hera.ugr.es/doi/14978994.pdf
Description
Summary:This paper reports the molecular and cytogenetic characterization of a HindIII family of satellite DNA in the bat species Pipistrellus pipistrellus. This satellite is organized in tandem repeats of 418 bp monomer units, and represents approximately 3 % of the whole genome. The consensus sequence from five cloned monomer units has an A–T content of 62.20%. We have found differences in the ladder pattern of bands between two populations of the same species. These differences are probably because of the absence of the target sites for the HindIII enzyme in most monomer units of one population, but not in the other. Fluorescent in situ hybridization (FISH) localized the satellite DNA in the pericentromeric regions of all autosomes and the X chromosome, but it was absent from the Y chromosome. Digestion of genomic DNAs with HpaII and its isoschizomer MspI demonstrated that these repetitive DNA sequences are not methylated. Other bat species were tested for the presence of this repetitive DNA. It was absent in five Vespertilionidae and one Rhinolophidae species, indicating that it could be a species/genus specific, repetitive DNA family.

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