KEY WORDS
Lipase B from Candida antarctica – kinetics – transesterification – organic solvent – diffusionnal limitation – solid/gas biocatalysis The influence of the addition of an extra component in a gaseous reaction medium, on the kinetics of alcoholysis of methyl propionate and n-propanol catalyzed by imm...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Text |
| Language: | English |
| Published: |
2008
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| Subjects: | |
| Online Access: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.372.8812 http://hal.archives-ouvertes.fr/docs/00/32/96/87/PDF/publi4.pdf |
| Summary: | Lipase B from Candida antarctica – kinetics – transesterification – organic solvent – diffusionnal limitation – solid/gas biocatalysis The influence of the addition of an extra component in a gaseous reaction medium, on the kinetics of alcoholysis of methyl propionate and n-propanol catalyzed by immobilized lipase B from Candida antarctica was studied in a continuous solid/gas reactor. In this reactor, the solid phase is composed of a packed enzymatic sample which is percolated by gaseous nitrogen, simultaneously carrying gaseous substrates and additional components to the enzyme while removing reaction products. The system permits to set thermodynamic activity of all gaseous components (substrates or not) independently at the desired values. This allows in particular to hal-00329687, version 1- 13 Oct 2008 study the influence of an extra added component at a constant thermodynamic activity value, contrary to classical solid/liquid system, which involves large variations of thermodynamic activity of added solvent, when performing full kinetic studies. Alcohol inhibition constant (KI) and methyl propionate and propanol dissociation constants (KMP and KP) have been determined in |
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