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An actinomycete strain was recovered from a pond where radon is known to be dissolved. A polyphasic study was undertaken to identify the new isolate. The 16S rRNA gene sequence of strain WA201 T showed closest similarity to the type strains of Micromonospora carbonacea (98?5 %) and Micromonospora ma...

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Bibliographic Details
Main Authors: Martha E. Trujillo, Carmen Fernández-molinero, Encarna Velázquez, Peter Schumann, Pedro F. Mateos, Eustoquio Martínez-molina
Other Authors: The Pennsylvania State University CiteSeerX Archives
Format: Text
Language:English
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Online Access:http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.332.5655
http://ijs.sgmjournals.org/content/55/2/877.full.pdf
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Summary:An actinomycete strain was recovered from a pond where radon is known to be dissolved. A polyphasic study was undertaken to identify the new isolate. The 16S rRNA gene sequence of strain WA201 T showed closest similarity to the type strains of Micromonospora carbonacea (98?5 %) and Micromonospora matsumotoense (98?1 %). The chemotaxonomic results confirmed the taxonomic position of the isolate in the genus Micromonospora. DNA–DNA relatedness values supported the classification of this isolate as a novel species. A number of physiological and biochemical tests were able to distinguish strain WA201 T from its closest phylogenetic neighbours. Therefore, it is proposed that isolate WA201 T (=DSM 44830 T =LMG 22229 T)be considered the type strain representing a novel species, Micromonospora mirobrigensis sp. nov. Representatives of the genus Micromonospora (Ørskov, 1923) are reported to inhabit soil, water, marine environments and sediments (Lüdemann & Brodsky, 1963; Kawamoto, 1989). Micromonospora endolithica, isolated from an extreme Antarctic sandstone environment, has been described recently (Hirsch et al., 2004), showing that