Archive Institutionnelle de l’Ifremer The oyster vasa-like gene: a specific marker of the germline in

Abstract: The vasa gene is a key determinant for germline formation in eukaryotes. This gene, highly conserved through evolution, encodes a RNA helicase protein member of the DEAD-box family. To understand the germline formation in oyster, we report here the isolation and the characterization of a v...

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Bibliographic Details
Main Authors: Crassostrea Gigas, Caroline Fabioux A, Stéphane Pouvreau A, Frédérique Le Roux C, Arnaud Huvet A
Other Authors: The Pennsylvania State University CiteSeerX Archives
Format: Text
Language:English
Published: 2004
Subjects:
Author Keywords
Oyster
Gametogenesis
Germline
vasa
DEAD-box
RNA helicase
Vasa
Crassostrea gigas
Online Access:http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.323.4161
http://archimer.ifremer.fr/doc/2004/publication-646.pdf
Description
Summary:Abstract: The vasa gene is a key determinant for germline formation in eukaryotes. This gene, highly conserved through evolution, encodes a RNA helicase protein member of the DEAD-box family. To understand the germline formation in oyster, we report here the isolation and the characterization of a vasa orthologue in Crassostrea gigas (Oyvlg). OyVLG contained the eight consensus domains of the DEAD-box including those providing RNA unwinding activity. The expression pattern of Oyvlg was examined in adult oyster tissues at different reproductive stages. Its expression was restricted to germline cells both in males and females, including germinal stem cells and auxiliary cells. The expression of Oyvlg, strongest in early germ cells, decreased as the maturation proceeded. These data and the evolutionary conservation observed suggested the role of Oyvlg in germline development. Oyvlg is the first germ cell specific marker in oyster and will be very useful in studies of oyster germline formation.

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