| Summary: | Strategy for improving extracellular lipolytic activities by a novel thermotolerant Staphylococcus sp. strain Slim Cherif * , Sami Mnif, Fatma Hadrich, Slim Abdelkafi and Sami Sayadi Background: Extracellular bacterial lipases received much attention for their substrate specificity and their ability to function under extreme environments (pH, temperature.). Many staphylococci produced lipases which were released into the culture medium. Reports of extracellular thermostable lipases from Staphylococcus sp. and active in alkaline conditions are not previously described. Results: This study focused on novel strategies to increase extracellular lipolytic enzyme production by a novel Staphylococcus sp. strain ESW. The microorganism needed neutral or alkaline pH values between 7.0 and 12.0 for growth. For pH values outside this range, cell growth seemed to be significantly inhibited. Staphylococcus sp. culture was able to grow within a wide temperature range (from 30 to 55°C). The presence of oils in the culture medium leaded to improvements in cells growth and lipolytic enzyme activity. On the other hand, although chemical surfactants leaded to an almost complete inhibition of growth and lipolytic enzyme production, their
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