ABSTRACT A method using liquid chromatography/tandem mass spectrometry (LC/MS/MS) was developed for the determination of 17α-ethynylestradiol in fish liver; a second method using LC/MS was developed for the determination of carbamazepine, diazepam, simvastatin, and oxybenzone in fish liver. The fish...
| Main Authors: | , , , , |
|---|---|
| Other Authors: | |
| Format: | Text |
| Language: | English |
| Subjects: | |
| Online Access: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1086.4995 http://ftp.sccwrp.org/pub/download/DOCUMENTS/AnnualReports/2009AnnualReport/AR09_291_303.pdf |
| Summary: | ABSTRACT A method using liquid chromatography/tandem mass spectrometry (LC/MS/MS) was developed for the determination of 17α-ethynylestradiol in fish liver; a second method using LC/MS was developed for the determination of carbamazepine, diazepam, simvastatin, and oxybenzone in fish liver. The fish liver samples were extracted and cleaned up by using liquid-liquid extraction and solid-phase extraction before the extracts were analyzed by LC/MS or LC/MS/MS with electrospray negative and positive ionization. Recoveries of the five target compounds from spiked catfish liver ranged between 72 ±2% and 100 ±3%. Quantification limits for the five compounds ranged between 4.2 and 12.3 ng/g (wet weight). Ten turbot (Pleuronichthys verticalis) liver samples were analyzed; levels of 17α-ethynylestradiol, carbamazepine, simvastatin, and oxybenzone were below the detection limits. Diazepam was detected in all 10 fish liver samples at concentrations ranging between 23 and 110 ng/g (wet weight). |
|---|