Production and Concentration of Monoacylglycerols Rich in Omega-3 Polyunsaturated Fatty Acids by Enzymatic Glycerolysis and Molecular Distillation

• Main Authors: Á G Solaesa, M T Sanz, M Falkeborg, S Beltrán, Z Guo
• Other Authors: The Pennsylvania State University CiteSeerX Archives
• Format: Text
• Language: English
• Subjects: Antarc*; Antarctica
• Online Access: http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1073.1406; http://riubu.ubu.es/bitstream/10259/4320/1/Garc%C3%ADa-FC_2016.pdf

Production and concentration of monoacylglycerols rich in omega-3 polyunsaturated fatty acids by enzymatic glycerolysis and molecular distillation (2016) Food Chemistry, 190, art. no. 17743, pp. 960-967 Abstract Production of monoacylglycerol (MAG) concentrate rich in omega 3 polyunsaturated fatty acids (n-3 PUFAs) was conducted through short path distillation (SPD) of an acylglyceride mixture produced by enzymatic glycerolysis. 67% yield of MAG was achieved in 2 h using sardine oil in a mole ratio of 1:3 with glycerol and 5% of immobilized Candida antarctica lipase B (Lipozyme 435) in tert-pentanol medium. For industrial interest and preservation of stability of the components, SPD under vacuum of 10 -3 mbar was used as an appropriate approach for fractionation of MAG operated in a UIC KDL 5 system. A stepwise SPD process was designed: the first distillation at evaporator temperature (T E ) of 110ºC was performed to remove completely the glycerol and most of FFA; and the second distillation at optimized T E 155ºC with feeding flow 1.0 mL/min; resulting in a stream distillate with MAG purity of 91% and 94% overall recovery of MAG. This work also demonstrated that SPD is able to concentrate n-3 PUFA in MAG form by distilling at proper T E e.g. 125ºC, where n-3 PUFAs will be concentrated in the residues. Moreover, this work mapped out a complete processing diagram for scalable production of n-3 PUFAs enriched MAG as potential food emulsifier and ingredient.