Selection of reference genes for RT-qPCR studies in blood of beluga whales ( Delphinapterus leucas ) Selection of reference genes for RT-qPCR studies in blood of beluga whales 2 (Delphinapterus leucas)

• Main Authors: Chen I, Wang J, Chou S, Wu Y Li, T, Leu M, Chang W, Yang, I-Hua Chen, Jiann-Hsiung Wang, Shih-Jen Chou, Yeong-Huey Wu, Tsung-Hsien Li, Ming-Yih Leu, Wen-Been Chang, Wei Cheng Yang, Ming -, Yih Leu, Wei-Cheng Yang
• Other Authors: The Pennsylvania State University CiteSeerX Archives
• Format: Text
• Language: English
• Subjects: Beluga; Beluga*; Delphinapterus leucas
• Online Access: http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1070.3502

Reverse transcription-quantitative PCR (RT-qPCR) is used for research in gene expression, and it is vital to choose appropriate housekeeping genes (HKGs) as reference genes to obtain correct result. The purpose of this study is to determine stably expressed HKGs in blood of beluga whales (Delphinapterus leucas) that can be the appropriate reference genes in relative quantification in gene expression research. Sixty blood samples were taken from 4 beluga whales. Thirteen candidate HKGs (ACTB, B2M, GAPDH, HPRT1, LDHB, PGK1, RPL4, RPL8, RPL18, RPS9, RPS18, TFRC, YWHAZ) were tested using RT-qPCR. 16 Reverse transcription-quantitative PCR (RT-qPCR) is used for research in gene expression, 17 and it is vital to choose appropriate housekeeping genes (HKGs) as reference genes to obtain 18 correct result. The purpose of this study is to determine stably expressed HKGs in blood of 19 beluga whales (Delphinapterus leucas) that can be the appropriate reference genes in relative 20 quantification in gene expression research. Sixty blood samples were taken from 4 beluga whales.