Molecular diagnosis of avian respiratory diseases in commercial broiler chicken flocks in province of Najaf, Iraq

ABSTRACT Avian influenza virus subtype 9 and Newcastle virus have been recognized as the most important pathogens in poultry. In Najaf governorate, Iraq, many chickens flocks suffered from high mortality (about 30-70%) with respiratory signs. In this study, trachea swabs and tissue specimens from 53...

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Bibliographic Details
Main Authors: Ali M Al-Mohana, Haider M Kadhim, Alaa H Al-Charrakh, Zainab Al-Habubi, Fadhil H Nasir, Samer A Al-Hilali, Zainab J Hadi
Other Authors: The Pennsylvania State University CiteSeerX Archives
Format: Text
Language:English
Subjects:
Avian flu
Online Access:http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1045.3073
http://www.uobabylon.edu.iq/uobcoleges/fileshare/articles/Avian%20Flu-Univ%20site.pdf
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Summary:ABSTRACT Avian influenza virus subtype 9 and Newcastle virus have been recognized as the most important pathogens in poultry. In Najaf governorate, Iraq, many chickens flocks suffered from high mortality (about 30-70%) with respiratory signs. In this study, trachea swabs and tissue specimens from 53 commercial broiler chicken flocks suffering from respiratory diseases were tested initially by rapid test for antigen influenza type A and by reverse transcription PCR for avian flu virus type A, H5, H7, H9, and Newcastle disease virus (NDV). The reverse transcription PCR results showed that 75% of these flocks were infected with both avian flu virus type H9 and NDV whereas 25% of them were infected only by H9. On the other hand all flocks were negative for subtypes H5, H7. Our data showed that above mentioned respiratory pathogens were the most important agents of respiratory diseases giving high mortality in broiler chicken in the area of the study.

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