| Summary: | Abstract Catalase is one of the most active antioxidant defence enzymes known for being highly cooperative with SOD and other H 2 O2 producers at high flux of hydrogen peroxide. In the present study, catalase activity was analyzed in liver samples of different animals both by gel activity staining method as well as spectrophotometric biochemical method. Five different animals like fish (Cirrhinus mrigala), amphibia (Bufo melanistictus.), reptile (Calotes versicolor.), bird (Gallus domesticus.), and mammal (Rattus rattus) from five different classes of vertebrates were taken for the study. Catalases from these animals differ in activities as shown by biochemical assay method and activity staining method.
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