Lipases/Acyltransferases for Lipid Modification in Aqueous Media

Lipases/acyltransferases are a special group of lipases with highly enhanced affinity for other nucleophiles than water compared to classical lipases. In the presence of a suitable acceptor, they catalyze acyltransfer reactions (such as alcoholysis, aminolysis, or perhydrolysis) much faster than hyd...

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Bibliographic Details
Main Authors: Subileau, Maeva, Jan, Anne Hélène, Dubreucq, Eric
Other Authors: Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)
Format: Book Part
Language:English
Published: HAL CCSD 2018
Subjects:
Acyltransfer
Alcoholysis
Biocatalysis
CAL-A from Moeziomyces antarcticus (Candida antarctica)
CpLIP2 from Candida parapsilosis
Structure/function
Transesterification
aqueous medium
acétyltransférase
hydrolase
milieu aqueux
lipase
[SDV.IDA]Life Sciences [q-bio]/Food engineering
Antarc*
Antarctica
antarcticus
Online Access:https://hal.science/hal-01918043
https://doi.org/10.1016/B978-0-12-813167-1.00003-7
Description
Summary:Lipases/acyltransferases are a special group of lipases with highly enhanced affinity for other nucleophiles than water compared to classical lipases. In the presence of a suitable acceptor, they catalyze acyltransfer reactions (such as alcoholysis, aminolysis, or perhydrolysis) much faster than hydrolysis even in aqueous media with a high thermodynamic activity of water (aW). This allows kinetically controlled reactions where the donor ester is fully converted into the ester product before any significant release of free fatty acids. This chapter will discuss the kinetic aspects of the competitive reactions catalyzed by lipases/acyltransferases, propose a model, and a procedure to quantify their acyltransferase character, present some applications, and give an overview of currently known lipases/acyltransferases and of the structural studies and protein engineering strategies that have aimed at further improving their catalytic properties or even at turning lipases into acyltransferases.

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