The immunomodulation of inducible hydrogen sulfide in Pacific oyster Crassostrea gigas

Hydrogen sulfide (H2S) is an important gasotransmitter, which plays indispensable roles in cardiovascular, nervous and immune systems of vertebrates. However, the information about the immunomodulaton of H2S in invertebrates is still very limited. In the present study, the temporal expression profil...

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Bibliographic Details
Published in:Developmental & Comparative Immunology
Main Authors: Sun, Zhibin, Wang, Lingling, Zhang, Tao, Zhou, Zhi, Jiang, Qiufen, Yi, Qilin, Yang, Chuanyan, Qiu, Limei, Song, Linsheng, Wang, LL (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China.
Format: Article in Journal/Newspaper
Language:English
Published: 2014
Subjects:
Crassostrea Gigas
Hydrogen Sulfide (H2s)
Cystathionine Gamma Lyase (Cse)
Immunity
Lps
Propargylglycine (Pag)
Immunology
Zoology
Pacific
Crassostrea gigas
Pacific oyster
Online Access:http://ir.qdio.ac.cn/handle/337002/24179
https://doi.org/10.1016/j.dci.2014.03.011
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Summary:Hydrogen sulfide (H2S) is an important gasotransmitter, which plays indispensable roles in cardiovascular, nervous and immune systems of vertebrates. However, the information about the immunomodulaton of H2S in invertebrates is still very limited. In the present study, the temporal expression profile of cystathionine gamma lyase in oyster Crassostrea gigas (CgCSE) was investigated after the oysters were stimulated by lipopolysaccharide. The expression levels of CgCSE mRNA transcripts in hemocytes increased significantly at 12 h (1.31-fold of the PBS group, P < 0.05) after LPS stimulation. The immunomodulation of inducible H2S in oyster was examined by monitoring the alterations of both cellular and humoral immune parameters in response to the stimulations of LPS, LPS + Na2S and LPS + propargylglycine (PAG). The total hemocyte counts (THC) and hemolymph PO activity increased significantly after LPS stimulation, and the increase could be further enhanced by adding PAG, while inhibited by appending Na2S. The phagocytosis activity of hemocytes was also increased firstly after LPS treatment, and the increase was enhanced by adding Na2S but inhibited after appending PAG. The anti-bacterial activity in hemolymph increased at 3 h post LPS treatment, and then decreased after adding PAG. The total SOD activity of hemolymph was also elevated at 6 h post LPS treatment, and the elevated activity was depressed by adding Na2S. These results collectively indicated that H2S might play crucial roles in the immune response of oyster via modulating the turnover and phagocytosis of hemocytes, and regulating the anti-bacterial activity and proPO activation in the hemolymph. (C) 2014 Elsevier Ltd. All rights reserved.

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