Green and eco-friendly method for the extraction of chitin from crab shells ( Chionoecetes opilio ) with Lactiplantibacillus plantarum and Pseudomonas aeruginosa

There are few reports on the extraction of chitin from crab shells using microbiological methods. In this work, the strain Lactiplantibacillus plantarum (LA01) for demineralization (DM) and the strain Pseudomonas aeruginosa (PS01) for deproteinization (DP) were screened from probiotic powders, soil,...

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Bibliographic Details
Published in:Biomass Conversion and Biorefinery
Main Authors: Zhou, Yi, Yang, Haoyue, Li, Guantian, Xing, Ronge, Liu, Song, Li, Kecheng, Li, Rongfeng, Yu, Huahua
Format: Report
Language:English
Published: SPRINGER HEIDELBERG 2024
Subjects:
Chitin extraction
Crab shells
Microbiological method
Antioxidant activity
Energy & Fuels
Engineering
Chemical
MOLECULAR-WEIGHT CHITIN
CHITOSAN
SHRIMP
WASTE
FERMENTATION
PRODUCTS
Chionoecetes opilio
Online Access:http://ir.qdio.ac.cn/handle/337002/186198
http://ir.qdio.ac.cn/handle/337002/186199
https://doi.org/10.1007/s13399-024-05970-y
Description
Summary:There are few reports on the extraction of chitin from crab shells using microbiological methods. In this work, the strain Lactiplantibacillus plantarum (LA01) for demineralization (DM) and the strain Pseudomonas aeruginosa (PS01) for deproteinization (DP) were screened from probiotic powders, soil, seawater, mud of aquaculture farm, seamount, and marine cold spring. After optimizing the fermentation conditions, PS01 followed by LA01 was chosen as the better inoculation sequence for two-step fermentation process to extract chitin from crab shells (Chionoecetes opilio). The chitin obtained by fermentation was analyzed by FTIR, XRD, SEM, and TGA. Results showed that 97.55 +/- 0.15% of calcium carbonate and 73.49 +/- 0.25% of protein were eliminated. The strong connection between protein and chitin in the crab shells was speculated as the reason of protein residues. A similar structure was observed in the crab chitin according to the results of structural analysis compared with commercial chitin, indicating the efficacy of microbiological methods used for chitin extraction from crab shells. The presence of porous and fibrous structure was observed in crab chitin according to the images of SEM. The organic acids in the supernatant of LA01 were mainly lactic acid (26,190.31 mg/L) and acetic acid (799.85 mg/L). The results of antioxidant activity assays showed that the fermentation supernatant possessed strong antioxidant activity, in which the scavenging activity of DPPH free radicals (96.50 +/- 1.68%) in LA01 was higher than that of 1.5 g/L ascorbic acid solution (96.18 +/- 0.43%), with 0.24 +/- 0.00 mg/mL of total phenolic and 7.54 +/- 0.31 mg/mL of exopolysaccharide.

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