Comparative mitochondrial genome analysis provides new insights into the classification of Modiolinae

BackgroundMitochondrial genomes have become a powerful tool for studying molecular genetics and phylogeny of mollusks. Currently, the position of Modiolinae within Mytilidae and the taxonomic and phylogenetic relationships within Modiolinae were still controversial. This study focuses on the complet...

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Bibliographic Details
Published in:Molecular Biology Reports
Main Authors: Zhu, Yi, Yan, Shaojing, Ma, Peizhen, Zhang, Yifei, Zuo, Chenxia, Ma, Xiaojie, Zhang, Zhen
Format: Report
Language:English
Published: SPRINGER 2024
Subjects:
Mytilidae
Modiolinae
Mitochondrial genome
Phylogeny
Genetic distance
Biochemistry & Molecular Biology
PHYLOGENETIC ANALYSIS
NUCLEOTIDE-SEQUENCE
MOLLUSCA MYTILIDAE
RNA GENE
DNA
MUSSEL
BIVALVIA
ORGANIZATION
REPLICATION
PERFORMANCE
Modiolus modiolus
Online Access:http://ir.qdio.ac.cn/handle/337002/185376
https://doi.org/10.1007/s11033-024-09767-0
Description
Summary:BackgroundMitochondrial genomes have become a powerful tool for studying molecular genetics and phylogeny of mollusks. Currently, the position of Modiolinae within Mytilidae and the taxonomic and phylogenetic relationships within Modiolinae were still controversial. This study focuses on the complete mitochondrial genomes of two species: Modiolus modulaides (R & ouml;ding, 1798) and Modiolus auriculatus Krauss, 1848, which have not been sequenced before.Methods and resultsWe assembled and characterized the mitochondrial genomes of M. modulaides and M. auriculatus and then analyzed the phylogenetic relationships. The mitochondrial genomes of M. modulaides and M. auriculatus were 15,422 bp and 16,027 bp, respectively. Both of them were composed of 36 functional genes, including 12 protein-coding genes, 22 transfer RNAs, and 2 ribosomal RNAs. All protein-coding genes showed A + T bias, positive GC skews, and negative AT skews in nucleotide composition. Phylogenetic analysis based on the mitochondrial genomes showed that Modiolinae and Bathymodiolinae clustered together to form a sister relationship. Seven Modiolinae species were divided into two clades: L1 (M. modulaides, M. auriculatus and Modiolus philippinarum Hanley, 1843) and L2 [Modiolus modiolus (Linnaeus, 1758), Modiolus kurilensis Bernard, 1983, Modiolus nipponicus (Oyama, 1950), and Modiolus comptus (Sowerby III, 1915)]. The divergence time of the two clades was approximately 105.75 Ma. Furthermore, the transfer RNA gene rearrangement, longer genetic distance, and greater genetic differentiation were confirmed between the L1 and L2 clades, as well as differences in the external characteristics of the shells of the two clades.ConclusionsBased on the molecular data, it was speculated that species from the L1 clade might belong to other genera or new genera. This study provides molecular information for further taxonomic and phylogenetic studies of Mytilidae.

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