DNA methylation-mediated energy metabolism provides new insight into the quality losses during oyster reproductive process

Excessive energy consumption during reproductive process seriously reduces the nutritive value of oysters. Significant reduction of meat quality was detected in condition index and glycogen content in postspawning oysters. In order to investigate the molecular mechanism underlying this variation in...

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Bibliographic Details
Published in:Aquaculture
Main Authors: Wang, Xinxing, Wei, Yuheng, Cong, Rihao, Wang, Wei, Qi, Haigang, Zhang, Guofan, Pan, Ying, Li, Li
Format: Report
Language:English
Published: ELSEVIER 2024
Subjects:
Oysters
Quality losses
Energy metabolism
DNA methylation
Reproductive process
AMPK signal pathway
Fisheries
Marine & Freshwater Biology
CRASSOSTREA-GIGAS
CONDITION INDEX
IDENTIFICATION
EPIGENETICS
PATHWAY
Crassostrea gigas
Online Access:http://ir.qdio.ac.cn/handle/337002/184785
https://doi.org/10.1016/j.aquaculture.2024.740595
Description
Summary:Excessive energy consumption during reproductive process seriously reduces the nutritive value of oysters. Significant reduction of meat quality was detected in condition index and glycogen content in postspawning oysters. In order to investigate the molecular mechanism underlying this variation in quality, a conjoint analysis of DNA methylation and RNA sequencing was conducted in this study. Oysters exhibited divergent gene expression profiles during reproduction, with a particular acceleration observed in glycogen and fatty acid metabolism related pathways in postspawning oysters, including glycolysis/gluconeogenesis, TCA cycle and fatty acid degradation. Changes in genomic DNA methylation between prespawning and postspawning oysters were also detected, with oysters after spawning exhibited increased methylation trend in promoter, genebody and downstream regions. Significantly, differently methylated genes were enriched in AMPK signal pathway, and differently methylated sites of AMPK alpha were identified. Combined with the gene expression variation in energy metabolism network dominated by AMPK, it can be inferred that DNA methylation could regulate energy metabolism homeostasis through influencing the gene expression of AMPK signal pathway. These findings suggested that DNA methylation-mediated energy metabolism plays a crucial role in regulating oyster glycogen content and meat quality during reproduction. This study revealed the epigenetic mechanism of quality variation during oyster reproduction for the first time.

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