Autophagy contributes to increase the content of intracellular free amino acids in hard clam (Mercenaria mercenaria) during prolonged exposure to hypersaline environments
Marine bivalves in intertidal zones and land-based seawater ponds are constantly subjected to a wide range of salinity fluctuations due to heavy rainfall, intense drought, and human activities. As osmoconformers, bivalves rely primarily on rapid release or accumulation of free amino acids (FAAs) for...
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Language: | English |
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2023
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Online Access: | http://ir.qdio.ac.cn/handle/337002/181619 http://ir.qdio.ac.cn/handle/337002/181620 https://doi.org/10.1016/j.marenvres.2023.106198 |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/181620 2024-05-12T08:02:47+00:00 Autophagy contributes to increase the content of intracellular free amino acids in hard clam (Mercenaria mercenaria) during prolonged exposure to hypersaline environments Zhou, Cong Yang, Mei-jie Hu, Zhi Zou, Yan Shi, Pu Li, Yong-ren Guo, Yong-jun Song, Hao Zhang, Tao 2023-11-01 http://ir.qdio.ac.cn/handle/337002/181619 http://ir.qdio.ac.cn/handle/337002/181620 https://doi.org/10.1016/j.marenvres.2023.106198 英语 eng ELSEVIER SCI LTD MARINE ENVIRONMENTAL RESEARCH http://ir.qdio.ac.cn/handle/337002/181619 http://ir.qdio.ac.cn/handle/337002/181620 doi:10.1016/j.marenvres.2023.106198 Hyposalinity Hypersalinity Transcriptome Autophagy Free amino acids Environmental Sciences & Ecology Marine & Freshwater Biology Toxicology Environmental Sciences OYSTER CRASSOSTREA-GIGAS PACIFIC OYSTER MYTILUS-GALLOPROVINCIALIS ENDOPLASMIC-RETICULUM MACOMA-BALTHICA STRESS EXPRESSION SALINITY ADAPTATION MECHANISMS 期刊论文 2023 ftchinacasciocas https://doi.org/10.1016/j.marenvres.2023.106198 2024-04-14T23:52:16Z Marine bivalves in intertidal zones and land-based seawater ponds are constantly subjected to a wide range of salinity fluctuations due to heavy rainfall, intense drought, and human activities. As osmoconformers, bivalves rely primarily on rapid release or accumulation of free amino acids (FAAs) for osmoregulation. Euryhaline bivalves are capable of withstanding hyposaline and hypersaline environments through regulation of physiology, metabolism, and gene expression. However, current understanding of the molecular mechanisms underlying osmoregulation and salinity adaptation in euryhaline bivalves remains largely limited. In this study, RNA-seq, WGCNA and flow cytometric analysis were performed to investigate the physiological responses of hard clams (Mercenaria mercenaria) to acute short-term hyposalinity (AL) and hypersalinity (AH), and chronic long-term hyposalinity (CL) and hypersalinity (CH) stress. We found that amino acids biosynthesis was significantly inhibited and aminoacyl-tRNA biosynthesis was augmented to decrease intracellular osmolarity during hypo saline exposure. Under CH, numerous autophagy-related genes (ATGs) were highly expressed, and the autophagy activity of gill cells were significantly up-regulated. A significant decrease in total FAAs content was observed in gills after NH4Cl treatment, indicating that autophagy was crucial for osmoregulation in hard clams during prolonged exposure to hypersaline environments. To prevent premature or unnecessary apoptosis, the expression of cathepsin L was inhibited under AL and AH, and inhibitors of apoptosis was augmented under CL and CH. Additionally, neuroendocrine regulation was involved in salinity adaption in hard clams. This study provides novel insights into the physiological responses of euryhaline marine bivalves to hyposaline and hypersaline environments. Report Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Marine Environmental Research 192 106198 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Hyposalinity Hypersalinity Transcriptome Autophagy Free amino acids Environmental Sciences & Ecology Marine & Freshwater Biology Toxicology Environmental Sciences OYSTER CRASSOSTREA-GIGAS PACIFIC OYSTER MYTILUS-GALLOPROVINCIALIS ENDOPLASMIC-RETICULUM MACOMA-BALTHICA STRESS EXPRESSION SALINITY ADAPTATION MECHANISMS |
spellingShingle |
Hyposalinity Hypersalinity Transcriptome Autophagy Free amino acids Environmental Sciences & Ecology Marine & Freshwater Biology Toxicology Environmental Sciences OYSTER CRASSOSTREA-GIGAS PACIFIC OYSTER MYTILUS-GALLOPROVINCIALIS ENDOPLASMIC-RETICULUM MACOMA-BALTHICA STRESS EXPRESSION SALINITY ADAPTATION MECHANISMS Zhou, Cong Yang, Mei-jie Hu, Zhi Zou, Yan Shi, Pu Li, Yong-ren Guo, Yong-jun Song, Hao Zhang, Tao Autophagy contributes to increase the content of intracellular free amino acids in hard clam (Mercenaria mercenaria) during prolonged exposure to hypersaline environments |
topic_facet |
Hyposalinity Hypersalinity Transcriptome Autophagy Free amino acids Environmental Sciences & Ecology Marine & Freshwater Biology Toxicology Environmental Sciences OYSTER CRASSOSTREA-GIGAS PACIFIC OYSTER MYTILUS-GALLOPROVINCIALIS ENDOPLASMIC-RETICULUM MACOMA-BALTHICA STRESS EXPRESSION SALINITY ADAPTATION MECHANISMS |
description |
Marine bivalves in intertidal zones and land-based seawater ponds are constantly subjected to a wide range of salinity fluctuations due to heavy rainfall, intense drought, and human activities. As osmoconformers, bivalves rely primarily on rapid release or accumulation of free amino acids (FAAs) for osmoregulation. Euryhaline bivalves are capable of withstanding hyposaline and hypersaline environments through regulation of physiology, metabolism, and gene expression. However, current understanding of the molecular mechanisms underlying osmoregulation and salinity adaptation in euryhaline bivalves remains largely limited. In this study, RNA-seq, WGCNA and flow cytometric analysis were performed to investigate the physiological responses of hard clams (Mercenaria mercenaria) to acute short-term hyposalinity (AL) and hypersalinity (AH), and chronic long-term hyposalinity (CL) and hypersalinity (CH) stress. We found that amino acids biosynthesis was significantly inhibited and aminoacyl-tRNA biosynthesis was augmented to decrease intracellular osmolarity during hypo saline exposure. Under CH, numerous autophagy-related genes (ATGs) were highly expressed, and the autophagy activity of gill cells were significantly up-regulated. A significant decrease in total FAAs content was observed in gills after NH4Cl treatment, indicating that autophagy was crucial for osmoregulation in hard clams during prolonged exposure to hypersaline environments. To prevent premature or unnecessary apoptosis, the expression of cathepsin L was inhibited under AL and AH, and inhibitors of apoptosis was augmented under CL and CH. Additionally, neuroendocrine regulation was involved in salinity adaption in hard clams. This study provides novel insights into the physiological responses of euryhaline marine bivalves to hyposaline and hypersaline environments. |
format |
Report |
author |
Zhou, Cong Yang, Mei-jie Hu, Zhi Zou, Yan Shi, Pu Li, Yong-ren Guo, Yong-jun Song, Hao Zhang, Tao |
author_facet |
Zhou, Cong Yang, Mei-jie Hu, Zhi Zou, Yan Shi, Pu Li, Yong-ren Guo, Yong-jun Song, Hao Zhang, Tao |
author_sort |
Zhou, Cong |
title |
Autophagy contributes to increase the content of intracellular free amino acids in hard clam (Mercenaria mercenaria) during prolonged exposure to hypersaline environments |
title_short |
Autophagy contributes to increase the content of intracellular free amino acids in hard clam (Mercenaria mercenaria) during prolonged exposure to hypersaline environments |
title_full |
Autophagy contributes to increase the content of intracellular free amino acids in hard clam (Mercenaria mercenaria) during prolonged exposure to hypersaline environments |
title_fullStr |
Autophagy contributes to increase the content of intracellular free amino acids in hard clam (Mercenaria mercenaria) during prolonged exposure to hypersaline environments |
title_full_unstemmed |
Autophagy contributes to increase the content of intracellular free amino acids in hard clam (Mercenaria mercenaria) during prolonged exposure to hypersaline environments |
title_sort |
autophagy contributes to increase the content of intracellular free amino acids in hard clam (mercenaria mercenaria) during prolonged exposure to hypersaline environments |
publisher |
ELSEVIER SCI LTD |
publishDate |
2023 |
url |
http://ir.qdio.ac.cn/handle/337002/181619 http://ir.qdio.ac.cn/handle/337002/181620 https://doi.org/10.1016/j.marenvres.2023.106198 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
MARINE ENVIRONMENTAL RESEARCH http://ir.qdio.ac.cn/handle/337002/181619 http://ir.qdio.ac.cn/handle/337002/181620 doi:10.1016/j.marenvres.2023.106198 |
op_doi |
https://doi.org/10.1016/j.marenvres.2023.106198 |
container_title |
Marine Environmental Research |
container_volume |
192 |
container_start_page |
106198 |
_version_ |
1798844921203916800 |