Proteomics reveals the changes in energy metabolism associated with reproduction in the clam Meretrix petechialis
Because reproduction requires a considerable energy investment that presents metabolic challenges to animals, there is growing scientific interest in the effects of reproduction on metabolism. Here, the proteome of the hepatopancreas from female and male clams (Meretrix petechialis) before and after...
| Published in: | Comparative Biochemistry and Physiology Part D: Genomics and Proteomics |
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| Main Authors: | , |
| Format: | Report |
| Language: | English |
| Published: |
ELSEVIER SCIENCE INC
2022
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| Subjects: | |
| Online Access: | http://ir.qdio.ac.cn/handle/337002/178767 https://doi.org/10.1016/j.cbd.2021.100954 |
| Summary: | Because reproduction requires a considerable energy investment that presents metabolic challenges to animals, there is growing scientific interest in the effects of reproduction on metabolism. Here, the proteome of the hepatopancreas from female and male clams (Meretrix petechialis) before and after spawning was obtained using iTRAQ-based proteome analysis. Forty-two DEPs and 37 DEPs were detected in the pre-and post-spawning comparison of females and males, respectively. KEGG pathway analysis was then performed to explore the function of the identified DEPs and the results showed that metabolic process was deeply affected by spawning. Carbohydrate metabolism was weakened after spawning, whereas protein and amino acid metabolism were enhanced. In addition, spawning induced more severe oxidative damage in females than in males, which may hinder muscle function in females. Finally, the total glucose and protein contents briefly increased after spawning and then recovered to the baseline level. Our results illustrate the overall differences and contribute to an improved understanding of the molecular mechanisms underlying energy changes in the clams during reproduction. |
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