The characterization of an interleukin-12 p35 homolog involved in the immune modulation of oyster Crassostrea gigas

Vertebrate interleukin-12 (IL-12) is a heterodimeric cytokine composing of two subunits (p35 and p40). In the present study, a p35-like subunit homolog of vertebrate IL-12 was identified from oyster Crassostrea gigas (designated as CgIL12p35L), with an open reading frame of 411 bp encoding a putativ...

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Bibliographic Details
Published in:Developmental & Comparative Immunology
Main Authors: Xin, Lusheng, Liu, Chang, Zhang, Huan, Qiu, Limei, Wang, Lingling, Song, Linsheng
Format: Report
Language:English
Published: ELSEVIER SCI LTD 2021
Subjects:
Crassostrea gigas
Cytokine
IL-12
IL-12 p35 subunit
Fisheries
Immunology
Veterinary Sciences
Zoology
Online Access:http://ir.qdio.ac.cn/handle/337002/177026
http://ir.qdio.ac.cn/handle/337002/177027
https://doi.org/10.1016/j.dci.2021.104145
Description
Summary:Vertebrate interleukin-12 (IL-12) is a heterodimeric cytokine composing of two subunits (p35 and p40). In the present study, a p35-like subunit homolog of vertebrate IL-12 was identified from oyster Crassostrea gigas (designated as CgIL12p35L), with an open reading frame of 411 bp encoding a putative peptide of 136 amino acids. There was a long four-helix chain in CgIL12p35L, which was similar as that in vertebrate IL-12 p35. Comparative genomic analysis showed that there were conservative kinds of syntenic genes flanked CgIL12p35L. The mRNA transcripts of CgIL12p35L were constitutively expressed in various tissues and its mRNA expression level in haemocytes increased significantly after bacteria challenge. The activity of haemolymph to eliminate bacteria from the oysters treated with recombinant CgIL12p35L protein (rCgIL12p35L) in vivo increased significantly. The results collectively indicated that the homolog of vertebrate IL-12 p35 subunit existed in oysters, and it was involved in immune defense against bacteria challenge.

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