Ca2+/Calmodulin-NOS/NO-TNFs Pathway Hallmarks the Inflammation Response of Oyster During Aerial Exposure
Aerial exposure (emersion) due to the periodical ebb and flow of tides is a major stressor for intertidal organisms and a key environmental factor in shaping their local communities. Oysters are among the most emersion-tolerant mollusk species and can survive for several days under aerial exposure....
Published in: | Frontiers in Marine Science |
---|---|
Main Authors: | , , , , , , , , |
Format: | Report |
Language: | English |
Published: |
FRONTIERS MEDIA SA
2021
|
Subjects: | |
Online Access: | http://ir.qdio.ac.cn/handle/337002/170089 https://doi.org/10.3389/fmars.2020.603825 |
id |
ftchinacasciocas:oai:ir.qdio.ac.cn:337002/170089 |
---|---|
record_format |
openpolar |
spelling |
ftchinacasciocas:oai:ir.qdio.ac.cn:337002/170089 2023-05-15T15:59:04+02:00 Ca2+/Calmodulin-NOS/NO-TNFs Pathway Hallmarks the Inflammation Response of Oyster During Aerial Exposure Chen, Hao Xin, Lusheng Wang, Lin Zhang, Huan Liu, Rui Wang, Hao Qiao, Xue Wang, Lingling Song, Linsheng 2021-01-11 http://ir.qdio.ac.cn/handle/337002/170089 https://doi.org/10.3389/fmars.2020.603825 英语 eng FRONTIERS MEDIA SA FRONTIERS IN MARINE SCIENCE http://ir.qdio.ac.cn/handle/337002/170089 doi:10.3389/fmars.2020.603825 intertidal zone calmodulin nitrite oxide tumor necrosis factor aerial exposure Environmental Sciences & Ecology Marine & Freshwater Biology Environmental Sciences NITRIC-OXIDE SYNTHASE INTRACELLULAR CALCIUM-CONCENTRATION CRASSOSTREA-GIGAS PHYSIOLOGICAL ADAPTATIONS TARGET RECOGNITION OXIDATIVE STRESS AIR EXPOSURE HEAT-SHOCK ACTIVATION 期刊论文 2021 ftchinacasciocas https://doi.org/10.3389/fmars.2020.603825 2022-06-27T05:43:33Z Aerial exposure (emersion) due to the periodical ebb and flow of tides is a major stressor for intertidal organisms and a key environmental factor in shaping their local communities. Oysters are among the most emersion-tolerant mollusk species and can survive for several days under aerial exposure. Noticeably, overwhelming inflammation responses could occur during the emersion stress. However, mechanisms beneath the activation and modulation of emersion-induced inflammation response have remained largely unknown. Ca2+ is an important intracellular second messenger that plays indispensable roles in inflammation response by cooperation with calmodulin (CaM) genes. Here, we showed that intracellular Ca2+ accumulates rapidly in oyster hemocytes during emersion stress along with the changes in the protein levels of three CaM genes, which function as intracellular sensors of Ca2+. As downstream effector of Ca2+/CaM complex, nitric oxide synthase (NOS) activity in hemocytes was enhanced during the emersion stress, facilitating a greater production of nitrite oxide (NO). Augmentation of NO concentration was associated with the increased mRNA expression levels of two oyster cytokines (CgTNFs) during aerial exposure. The robust accumulation of cytokines and severe injury of tissues in oysters have been regarded as potential cause and marker of their death in prolonged emersion stress. Here, both the expression levels of CgTNFs and the tissue injuries of oysters were attenuated when Ca2+/CaM complex or NOS activity were repressed in vivo during the emersion stress. These findings indicate that Ca2+/CaM-NOS/NO-CgTNFs pathway is critically involved in the emersion-induced inflammation response in oysters and plays a role in the resistance against long-term aerial exposure. Report Crassostrea gigas Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Frontiers in Marine Science 7 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
intertidal zone calmodulin nitrite oxide tumor necrosis factor aerial exposure Environmental Sciences & Ecology Marine & Freshwater Biology Environmental Sciences NITRIC-OXIDE SYNTHASE INTRACELLULAR CALCIUM-CONCENTRATION CRASSOSTREA-GIGAS PHYSIOLOGICAL ADAPTATIONS TARGET RECOGNITION OXIDATIVE STRESS AIR EXPOSURE HEAT-SHOCK ACTIVATION |
spellingShingle |
intertidal zone calmodulin nitrite oxide tumor necrosis factor aerial exposure Environmental Sciences & Ecology Marine & Freshwater Biology Environmental Sciences NITRIC-OXIDE SYNTHASE INTRACELLULAR CALCIUM-CONCENTRATION CRASSOSTREA-GIGAS PHYSIOLOGICAL ADAPTATIONS TARGET RECOGNITION OXIDATIVE STRESS AIR EXPOSURE HEAT-SHOCK ACTIVATION Chen, Hao Xin, Lusheng Wang, Lin Zhang, Huan Liu, Rui Wang, Hao Qiao, Xue Wang, Lingling Song, Linsheng Ca2+/Calmodulin-NOS/NO-TNFs Pathway Hallmarks the Inflammation Response of Oyster During Aerial Exposure |
topic_facet |
intertidal zone calmodulin nitrite oxide tumor necrosis factor aerial exposure Environmental Sciences & Ecology Marine & Freshwater Biology Environmental Sciences NITRIC-OXIDE SYNTHASE INTRACELLULAR CALCIUM-CONCENTRATION CRASSOSTREA-GIGAS PHYSIOLOGICAL ADAPTATIONS TARGET RECOGNITION OXIDATIVE STRESS AIR EXPOSURE HEAT-SHOCK ACTIVATION |
description |
Aerial exposure (emersion) due to the periodical ebb and flow of tides is a major stressor for intertidal organisms and a key environmental factor in shaping their local communities. Oysters are among the most emersion-tolerant mollusk species and can survive for several days under aerial exposure. Noticeably, overwhelming inflammation responses could occur during the emersion stress. However, mechanisms beneath the activation and modulation of emersion-induced inflammation response have remained largely unknown. Ca2+ is an important intracellular second messenger that plays indispensable roles in inflammation response by cooperation with calmodulin (CaM) genes. Here, we showed that intracellular Ca2+ accumulates rapidly in oyster hemocytes during emersion stress along with the changes in the protein levels of three CaM genes, which function as intracellular sensors of Ca2+. As downstream effector of Ca2+/CaM complex, nitric oxide synthase (NOS) activity in hemocytes was enhanced during the emersion stress, facilitating a greater production of nitrite oxide (NO). Augmentation of NO concentration was associated with the increased mRNA expression levels of two oyster cytokines (CgTNFs) during aerial exposure. The robust accumulation of cytokines and severe injury of tissues in oysters have been regarded as potential cause and marker of their death in prolonged emersion stress. Here, both the expression levels of CgTNFs and the tissue injuries of oysters were attenuated when Ca2+/CaM complex or NOS activity were repressed in vivo during the emersion stress. These findings indicate that Ca2+/CaM-NOS/NO-CgTNFs pathway is critically involved in the emersion-induced inflammation response in oysters and plays a role in the resistance against long-term aerial exposure. |
format |
Report |
author |
Chen, Hao Xin, Lusheng Wang, Lin Zhang, Huan Liu, Rui Wang, Hao Qiao, Xue Wang, Lingling Song, Linsheng |
author_facet |
Chen, Hao Xin, Lusheng Wang, Lin Zhang, Huan Liu, Rui Wang, Hao Qiao, Xue Wang, Lingling Song, Linsheng |
author_sort |
Chen, Hao |
title |
Ca2+/Calmodulin-NOS/NO-TNFs Pathway Hallmarks the Inflammation Response of Oyster During Aerial Exposure |
title_short |
Ca2+/Calmodulin-NOS/NO-TNFs Pathway Hallmarks the Inflammation Response of Oyster During Aerial Exposure |
title_full |
Ca2+/Calmodulin-NOS/NO-TNFs Pathway Hallmarks the Inflammation Response of Oyster During Aerial Exposure |
title_fullStr |
Ca2+/Calmodulin-NOS/NO-TNFs Pathway Hallmarks the Inflammation Response of Oyster During Aerial Exposure |
title_full_unstemmed |
Ca2+/Calmodulin-NOS/NO-TNFs Pathway Hallmarks the Inflammation Response of Oyster During Aerial Exposure |
title_sort |
ca2+/calmodulin-nos/no-tnfs pathway hallmarks the inflammation response of oyster during aerial exposure |
publisher |
FRONTIERS MEDIA SA |
publishDate |
2021 |
url |
http://ir.qdio.ac.cn/handle/337002/170089 https://doi.org/10.3389/fmars.2020.603825 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
FRONTIERS IN MARINE SCIENCE http://ir.qdio.ac.cn/handle/337002/170089 doi:10.3389/fmars.2020.603825 |
op_doi |
https://doi.org/10.3389/fmars.2020.603825 |
container_title |
Frontiers in Marine Science |
container_volume |
7 |
_version_ |
1766394844496789504 |