A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of OysterCrassostrea gigas

Cluster of differentiation 63 (CD63), a four-transmembrane glycoprotein in the subfamily of tetraspanin, has been widely recognized as a gateway from the infection of foreign invaders to the immune defense of hosts. Its role in Pacific oysterCrassostrea gigasis, however, yet to be discovered. This w...

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Bibliographic Details
Published in:Frontiers in Immunology
Main Authors: Liu, Conghui, Yang, Chuanyan, Wang, Mengqiang, Jiang, Shuai, Yi, Qilin, Wang, Weilin, Wang, Lingling, Song, Linsheng
Format: Report
Language:English
Published: FRONTIERS MEDIA SA 2020
Subjects:
tetraspanin
phagosomes recruitment
receptor
innate immune response
Crassostrea gigas
Immunology
C-TYPE LECTIN
ARGOPECTEN-IRRADIANS
MEMBRANE-PROTEINS
CD81
LIPOPOLYSACCHARIDE
ACTIVATION
EXPRESSION
OYSTER
Pacific
Online Access:http://ir.qdio.ac.cn/handle/337002/168179
https://doi.org/10.3389/fimmu.2020.01379
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Summary:Cluster of differentiation 63 (CD63), a four-transmembrane glycoprotein in the subfamily of tetraspanin, has been widely recognized as a gateway from the infection of foreign invaders to the immune defense of hosts. Its role in Pacific oysterCrassostrea gigasis, however, yet to be discovered. This work makes contributions by identifyingCgCD63H, a CD63 homolog with four transmembrane domains and one conservative CCG motif, and establishing its role as a receptor that participates in immune recognition and hemocyte phagocytosis. The presence ofCgCD63H messenger RNA (mRNA) in hepatopancreas, labial palps, gill, and hemocytes is confirmed. The expression level of mRNA in hemocytes is found significantly (p< 0.01) upregulated after the injection ofVibrio splendidus.CgCD63H protein, typically distributed over the plasma membrane of oyster hemocytes, is recruited to theYarrowia lipolytica-containing phagosomes after the stimulation ofY. lipolytica. The recombinantCgCD63H protein expresses binding capacity to glucan (GLU), peptidoglycan (PGN), and lipopolysaccharide (LPS) in the presence of lyophilized hemolymph. The phagocytic rate of hemocytes towardV. splendidusandY. lipolyticais significantly inhibited (p< 0.01) after incubation with anti-CgCD63H antibody. Our work further suggests thatCgCD63H functions as a receptor involved in the immune recognition and hemocyte phagocytosis against invading pathogen, which can be a marker candidate for the hemocyte typing inC. gigas.

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