Overexpression of T-bet, GATA-3 and TGF-ss Induces IFN-gamma, IL-4/13A, and IL-17A Expression in Atlantic Salmon
The overexpression of GATA-3, T-bet and TGF-ss may theoretically induce IL-4/A, IFN-gamma and IL-17A expression, respectively. Whether this also applies to fish is not yet known. The plasmid vectors encoding reporter gene (RFP)-tagged T-bet, GATA-3 and TGF-ss were used as overexpression tools, trans...
Published in: | Biology |
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Main Authors: | , , , , , , |
Format: | Report |
Language: | English |
Published: |
MDPI
2020
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Subjects: | |
Online Access: | http://ir.qdio.ac.cn/handle/337002/167436 http://ir.qdio.ac.cn/handle/337002/167437 https://doi.org/10.3390/biology9040082 |
Summary: | The overexpression of GATA-3, T-bet and TGF-ss may theoretically induce IL-4/A, IFN-gamma and IL-17A expression, respectively. Whether this also applies to fish is not yet known. The plasmid vectors encoding reporter gene (RFP)-tagged T-bet, GATA-3 and TGF-ss were used as overexpression tools, transfected into cells or injected intramuscularly to monitor the expression of IFN-gamma, IL-4/13A and IL-17A. In addition, the fish were either experimentally challenged with Vibrio anguillarum (VA group) or Piscirickettsia salmonis (PS group). The reporter gene (RFP) inserted upstream of the GATA-3, T-bet and TGF-ss genes, was observed in muscle cell nuclei and in inflammatory cells after intramuscular (i.m.) injection. PS group: following the injection of GATA-3 and T-bet-encoding plasmids, the expression of GATA-3 and T-bet was high at the injection site. The spleen expression of IFN-gamma, following the injection of a T-bet-encoding plasmid, was significantly higher on day 2. VA group: The T-bet and GATA-3-overexpressing fish expressed high T-bet and GATA-3 mRNA levels in the muscles and on day 4 post-challenge. The expression of TGF-ss in the muscles of fish injected with TGF-ss-encoding plasmids was significantly higher on days 7 (8 days pre-challenge) and 19 (4 days after challenge). The protective effects of the overexpression of T-bet, GATA-3 and TGF-ss on both bacterial infections were negligible. |
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