Molecular cloning and transcriptional regulation of an allograft inflammatory factor-1 (AIF-1) in Zhikong scallop Chlamys farreri

The allograft inflammatory factor-1 (AIF-1) is one of the key factors associated with inflammatory response. In the present study, the full-length cDNA of AIF-1 was identified from Zhikong scallop Chlamys farreri (named as CfAIF-1) by EST (expressed sequence tag) analysis and RACE (rapid-amplificati...

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Bibliographic Details
Published in:Gene
Main Authors: Wang, Jingjing, Zhang, Huan, Wang, Lingling, Qiu, Limei, Yue, Feng, Yang, Chuanyan, Song, Linsheng, Song, LS
Format: Article in Journal/Newspaper
Language:English
Published: 2013
Subjects:
Allograft Inflammatory Factor-1
Chlamys Farreri
Immune Response
Bacteria Challenge
Pamp Stimulation
Larval Development
Genetics & Heredity
Science & Technology
Life Sciences & Biomedicine
IMMUNE-RELATED GENES
BACTERIAL CHALLENGE
CRASSOSTREA-GIGAS
TISSUE-INJURY
EXPRESSION
CYTOKINE
ONTOGENY
IDENTIFICATION
PROLIFERATION
MACROPHAGES
Crassostrea gigas
Online Access:http://ir.qdio.ac.cn/handle/337002/16605
https://doi.org/10.1016/j.gene.2013.08.050
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Summary:The allograft inflammatory factor-1 (AIF-1) is one of the key factors associated with inflammatory response. In the present study, the full-length cDNA of AIF-1 was identified from Zhikong scallop Chlamys farreri (named as CfAIF-1) by EST (expressed sequence tag) analysis and RACE (rapid-amplification of cDNA ends) approaches. The cDNA of CfAIF-1 consisted of a 5-terminal untranslated region (UTR) of 58 bp, a 3-UTR of 607 bp with a poly (A) tail, and an open reading frame (ORF) of 468 bp encoding a polypeptide of 155 amino acids with the putative molecular mass of 17.8 kDa. There was an EF hand Ca2+-binding motif in the deduced amino acid sequence of CfAIF-1 which was conserved in other AIF-1s. CfAIF-1 shared closer phylogenetic relationship with invertebrate counterparts than vertebrate. The mRNA transcripts of CfAIF-1 were dominantly expressed in hepatopancreas, hemocytes and adductor. During scallop ontogenesis, the CfAIF-1 mRNA was expressed at a low level at early developmental stages from eggs to blastula, and then increased significantly from gastruta to late veliger larvae (P < 0.05). Moreover, the mRNA expression levels of CfAIF-1 in the hemocytes of adult scallop were significantly up-regulated during 12-48 h after LPS, PGN and poly I:C stimulation (P < 0.01), but there was no significant fluctuation detected after glucan stimulation. Furthermore, the challenge of bacteria Vibrio anguillarum remarkably induced the mRNA expression of CfAIF-1 in hemocytes at 6 h (P < 0.05) and 12 h (P < 0.01). All these results collectively indicated that CfAIF-1 might be involved in the immune response during the ontogenesis and contribute to the defense against microbe infection in scallops. (c) 2013 Elsevier B.V. All rights reserved. The allograft inflammatory factor-1 (AIF-1) is one of the key factors associated with inflammatory response. In the present study, the full-length cDNA of AIF-1 was identified from Zhikong scallop Chlamys farreri (named as CfAIF-1) by EST (expressed sequence tag) analysis ...

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