Characterization of a c-type lysozyme of Scophthalmus maximus: Expression, activity, and antibacterial effect
Lysozyme is a key component of the innate immune system and plays an important role in antibacterial infection. In this study, we analyzed the expression and activity of a chicken-type (c-type) lysozyme (named SmLysC) from turbot (Scophthalmus maximus). SmLysC is composed of 143 residues and shares...
Published in: | Fish & Shellfish Immunology |
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Language: | English |
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2013
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Online Access: | http://ir.qdio.ac.cn/handle/337002/16538 https://doi.org/10.1016/j.fsi.2012.10.007 |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/16538 2023-05-15T18:15:41+02:00 Characterization of a c-type lysozyme of Scophthalmus maximus: Expression, activity, and antibacterial effect Yu, Lan-ping Sun, Bo-guang Li, Jun Sun, Li Sun, L 2013 http://ir.qdio.ac.cn/handle/337002/16538 https://doi.org/10.1016/j.fsi.2012.10.007 英语 eng FISH & SHELLFISH IMMUNOLOGY Yu, Lan-ping; Sun, Bo-guang; Li, Jun; Sun, Li.Characterization of a c-type lysozyme of Scophthalmus maximus: Expression, activity, and antibacterial effect,FISH & SHELLFISH IMMUNOLOGY,2013,34(1):46-54 http://ir.qdio.ac.cn/handle/337002/16538 doi:10.1016/j.fsi.2012.10.007 6 C-type Lysozyme Scophthalmus Maximus Bacteriolytic Catalytic Site Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences Science & Technology Life Sciences & Biomedicine GOOSE-TYPE LYSOZYME MOLECULAR CHARACTERIZATION IMMUNOPROTECTIVE ANALYSIS VACCINE CANDIDATE CDNA IDENTIFICATION PHYLOGENY INFECTION PATTERN ANTIGEN Article 期刊论文 2013 ftchinacasciocas https://doi.org/10.1016/j.fsi.2012.10.007 2022-06-27T05:35:40Z Lysozyme is a key component of the innate immune system and plays an important role in antibacterial infection. In this study, we analyzed the expression and activity of a chicken-type (c-type) lysozyme (named SmLysC) from turbot (Scophthalmus maximus). SmLysC is composed of 143 residues and shares 67-90% overall sequence identities with the c-type lysozymes of a number of teleost fish. SmLysC possesses a typical c-type lysozyme domain, which contains the conserved residues E50 and D67 that form the putative catalytic site. SmLysC expression was detected, in increasing order, in head kidney, gill, heart, muscle, brain, spleen, blood, and liver. Bacterial infection caused significant inductions of SmLysC expression in head kidney, spleen, and liver in a time-dependent manner. Immunoblot analysis indicated that SmLysC has a subcellular localization in the extracellular milieu. Recombinant SmLysC (rSmLysC) was able to bind to bacterial cells and inhibit bacterial growth. Enzyme assay showed that the optimal temperature and pH of rSmLysC were 37 degrees C and pH 6.0 respectively. In contrast to rSmLysC, the mutant protein rSmLysCM1, which bears alanine substitutions at E50 and D67, displayed drastically reduced bacteriolytic activity. rSmLysC was able to inhibit the growth of several fish bacterial pathogens in a manner that depended on the dose of the protein; however, Gram-positive bacteria were in general more sensitive to rSmLysC than Gram-negative bacteria. Together these results indicate that SmLysC is a functional lysozyme that is likely to participate in innate immune defense against extracellular bacterial pathogens, in particular those of Gram-positive nature. (c) 2012 Elsevier Ltd. All rights reserved. Lysozyme is a key component of the innate immune system and plays an important role in antibacterial infection. In this study, we analyzed the expression and activity of a chicken-type (c-type) lysozyme (named SmLysC) from turbot (Scophthalmus maximus). SmLysC is composed of 143 residues and shares 67-90% ... Article in Journal/Newspaper Scophthalmus maximus Turbot Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Fish & Shellfish Immunology 34 1 46 54 |
institution |
Open Polar |
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Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
C-type Lysozyme Scophthalmus Maximus Bacteriolytic Catalytic Site Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences Science & Technology Life Sciences & Biomedicine GOOSE-TYPE LYSOZYME MOLECULAR CHARACTERIZATION IMMUNOPROTECTIVE ANALYSIS VACCINE CANDIDATE CDNA IDENTIFICATION PHYLOGENY INFECTION PATTERN ANTIGEN |
spellingShingle |
C-type Lysozyme Scophthalmus Maximus Bacteriolytic Catalytic Site Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences Science & Technology Life Sciences & Biomedicine GOOSE-TYPE LYSOZYME MOLECULAR CHARACTERIZATION IMMUNOPROTECTIVE ANALYSIS VACCINE CANDIDATE CDNA IDENTIFICATION PHYLOGENY INFECTION PATTERN ANTIGEN Yu, Lan-ping Sun, Bo-guang Li, Jun Sun, Li Sun, L Characterization of a c-type lysozyme of Scophthalmus maximus: Expression, activity, and antibacterial effect |
topic_facet |
C-type Lysozyme Scophthalmus Maximus Bacteriolytic Catalytic Site Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences Science & Technology Life Sciences & Biomedicine GOOSE-TYPE LYSOZYME MOLECULAR CHARACTERIZATION IMMUNOPROTECTIVE ANALYSIS VACCINE CANDIDATE CDNA IDENTIFICATION PHYLOGENY INFECTION PATTERN ANTIGEN |
description |
Lysozyme is a key component of the innate immune system and plays an important role in antibacterial infection. In this study, we analyzed the expression and activity of a chicken-type (c-type) lysozyme (named SmLysC) from turbot (Scophthalmus maximus). SmLysC is composed of 143 residues and shares 67-90% overall sequence identities with the c-type lysozymes of a number of teleost fish. SmLysC possesses a typical c-type lysozyme domain, which contains the conserved residues E50 and D67 that form the putative catalytic site. SmLysC expression was detected, in increasing order, in head kidney, gill, heart, muscle, brain, spleen, blood, and liver. Bacterial infection caused significant inductions of SmLysC expression in head kidney, spleen, and liver in a time-dependent manner. Immunoblot analysis indicated that SmLysC has a subcellular localization in the extracellular milieu. Recombinant SmLysC (rSmLysC) was able to bind to bacterial cells and inhibit bacterial growth. Enzyme assay showed that the optimal temperature and pH of rSmLysC were 37 degrees C and pH 6.0 respectively. In contrast to rSmLysC, the mutant protein rSmLysCM1, which bears alanine substitutions at E50 and D67, displayed drastically reduced bacteriolytic activity. rSmLysC was able to inhibit the growth of several fish bacterial pathogens in a manner that depended on the dose of the protein; however, Gram-positive bacteria were in general more sensitive to rSmLysC than Gram-negative bacteria. Together these results indicate that SmLysC is a functional lysozyme that is likely to participate in innate immune defense against extracellular bacterial pathogens, in particular those of Gram-positive nature. (c) 2012 Elsevier Ltd. All rights reserved. Lysozyme is a key component of the innate immune system and plays an important role in antibacterial infection. In this study, we analyzed the expression and activity of a chicken-type (c-type) lysozyme (named SmLysC) from turbot (Scophthalmus maximus). SmLysC is composed of 143 residues and shares 67-90% ... |
format |
Article in Journal/Newspaper |
author |
Yu, Lan-ping Sun, Bo-guang Li, Jun Sun, Li Sun, L |
author_facet |
Yu, Lan-ping Sun, Bo-guang Li, Jun Sun, Li Sun, L |
author_sort |
Yu, Lan-ping |
title |
Characterization of a c-type lysozyme of Scophthalmus maximus: Expression, activity, and antibacterial effect |
title_short |
Characterization of a c-type lysozyme of Scophthalmus maximus: Expression, activity, and antibacterial effect |
title_full |
Characterization of a c-type lysozyme of Scophthalmus maximus: Expression, activity, and antibacterial effect |
title_fullStr |
Characterization of a c-type lysozyme of Scophthalmus maximus: Expression, activity, and antibacterial effect |
title_full_unstemmed |
Characterization of a c-type lysozyme of Scophthalmus maximus: Expression, activity, and antibacterial effect |
title_sort |
characterization of a c-type lysozyme of scophthalmus maximus: expression, activity, and antibacterial effect |
publishDate |
2013 |
url |
http://ir.qdio.ac.cn/handle/337002/16538 https://doi.org/10.1016/j.fsi.2012.10.007 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
FISH & SHELLFISH IMMUNOLOGY Yu, Lan-ping; Sun, Bo-guang; Li, Jun; Sun, Li.Characterization of a c-type lysozyme of Scophthalmus maximus: Expression, activity, and antibacterial effect,FISH & SHELLFISH IMMUNOLOGY,2013,34(1):46-54 http://ir.qdio.ac.cn/handle/337002/16538 doi:10.1016/j.fsi.2012.10.007 |
op_rights |
6 |
op_doi |
https://doi.org/10.1016/j.fsi.2012.10.007 |
container_title |
Fish & Shellfish Immunology |
container_volume |
34 |
container_issue |
1 |
container_start_page |
46 |
op_container_end_page |
54 |
_version_ |
1766188861806870528 |