Characterization of a c-type lysozyme of Scophthalmus maximus: Expression, activity, and antibacterial effect

Lysozyme is a key component of the innate immune system and plays an important role in antibacterial infection. In this study, we analyzed the expression and activity of a chicken-type (c-type) lysozyme (named SmLysC) from turbot (Scophthalmus maximus). SmLysC is composed of 143 residues and shares...

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Bibliographic Details
Published in:Fish & Shellfish Immunology
Main Authors: Yu, Lan-ping, Sun, Bo-guang, Li, Jun, Sun, Li, Sun, L
Format: Article in Journal/Newspaper
Language:English
Published: 2013
Subjects:
C-type Lysozyme
Scophthalmus Maximus
Bacteriolytic
Catalytic Site
Fisheries
Immunology
Marine & Freshwater Biology
Veterinary Sciences
Science & Technology
Life Sciences & Biomedicine
GOOSE-TYPE LYSOZYME
MOLECULAR CHARACTERIZATION
IMMUNOPROTECTIVE ANALYSIS
VACCINE CANDIDATE
CDNA
IDENTIFICATION
PHYLOGENY
INFECTION
PATTERN
ANTIGEN
Scophthalmus maximus
Turbot
Online Access:http://ir.qdio.ac.cn/handle/337002/16538
https://doi.org/10.1016/j.fsi.2012.10.007
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Summary:Lysozyme is a key component of the innate immune system and plays an important role in antibacterial infection. In this study, we analyzed the expression and activity of a chicken-type (c-type) lysozyme (named SmLysC) from turbot (Scophthalmus maximus). SmLysC is composed of 143 residues and shares 67-90% overall sequence identities with the c-type lysozymes of a number of teleost fish. SmLysC possesses a typical c-type lysozyme domain, which contains the conserved residues E50 and D67 that form the putative catalytic site. SmLysC expression was detected, in increasing order, in head kidney, gill, heart, muscle, brain, spleen, blood, and liver. Bacterial infection caused significant inductions of SmLysC expression in head kidney, spleen, and liver in a time-dependent manner. Immunoblot analysis indicated that SmLysC has a subcellular localization in the extracellular milieu. Recombinant SmLysC (rSmLysC) was able to bind to bacterial cells and inhibit bacterial growth. Enzyme assay showed that the optimal temperature and pH of rSmLysC were 37 degrees C and pH 6.0 respectively. In contrast to rSmLysC, the mutant protein rSmLysCM1, which bears alanine substitutions at E50 and D67, displayed drastically reduced bacteriolytic activity. rSmLysC was able to inhibit the growth of several fish bacterial pathogens in a manner that depended on the dose of the protein; however, Gram-positive bacteria were in general more sensitive to rSmLysC than Gram-negative bacteria. Together these results indicate that SmLysC is a functional lysozyme that is likely to participate in innate immune defense against extracellular bacterial pathogens, in particular those of Gram-positive nature. (c) 2012 Elsevier Ltd. All rights reserved. Lysozyme is a key component of the innate immune system and plays an important role in antibacterial infection. In this study, we analyzed the expression and activity of a chicken-type (c-type) lysozyme (named SmLysC) from turbot (Scophthalmus maximus). SmLysC is composed of 143 residues and shares 67-90% ...

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