Functional relationship between CgMyD88-1 and CgMyD88-2 in the Pacific oyster
MyD88 is a universal adapter protein for the Toll-like receptor/interleukin-1 receptor (TLR/IL-1R) signaling pathway. Since invertebrates are believed to lack MyD88-independent pathways, MyD88 appears more critical in oyster TLR signaling pathway. In the Pacific oyster (Crassostrea gigas), two compl...
Published in: | Fish & Shellfish Immunology |
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Main Authors: | , , , , , |
Format: | Report |
Language: | English |
Published: |
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
2020
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Subjects: | |
Online Access: | http://ir.qdio.ac.cn/handle/337002/164484 http://ir.qdio.ac.cn/handle/337002/164485 https://doi.org/10.1016/j.fsi.2019.10.060 |
Summary: | MyD88 is a universal adapter protein for the Toll-like receptor/interleukin-1 receptor (TLR/IL-1R) signaling pathway. Since invertebrates are believed to lack MyD88-independent pathways, MyD88 appears more critical in oyster TLR signaling pathway. In the Pacific oyster (Crassostrea gigas), two complete paralogues, named as CgMyD88-1 and CgMyD88-2, have been identified. In the current study, we indicated that CgMyD88-1 and CgMyD88-2 might act synergistically to increase the efficiency of immune signaling by activating NF-kappa B transcription factor. However, we found that upon stimulation with lipopolysaccharide (LPS) or polyinosinic:polycytidylic acid [poly (I:C)], CgMyD88-1 and CgMyD88-2 show differences in their response: CgMyD88-1 accumulated as large spots in the cytoplasm, while CgMyD88-2 assembled in the cytoplasm and in the membrane. Our results support the theory that expansion of these immune genes is associated with functional diversity. |
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