Functional relationship between CgMyD88-1 and CgMyD88-2 in the Pacific oyster

• Published in: Fish & Shellfish Immunology
• Main Authors: Tang, Xueying, Huang, Baoyu, Lin, Siheng, Wang, Wei, Zhang, Guofan, Li, Li
• Format: Report
• Language: English
• Published: ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD 2020
• Subjects: Crassostrea gigas; Innate immunity; MyD88; Functional diversity; Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences; FACTOR 88 MYD88; IDENTIFICATION; GENOME; Pacific; Pacific oyster
• Online Access: http://ir.qdio.ac.cn/handle/337002/164483; https://doi.org/10.1016/j.fsi.2019.10.060

MyD88 is a universal adapter protein for the Toll-like receptor/interleukin-1 receptor (TLR/IL-1R) signaling pathway. Since invertebrates are believed to lack MyD88-independent pathways, MyD88 appears more critical in oyster TLR signaling pathway. In the Pacific oyster (Crassostrea gigas), two complete paralogues, named as CgMyD88-1 and CgMyD88-2, have been identified. In the current study, we indicated that CgMyD88-1 and CgMyD88-2 might act synergistically to increase the efficiency of immune signaling by activating NF-kappa B transcription factor. However, we found that upon stimulation with lipopolysaccharide (LPS) or polyinosinic:polycytidylic acid [poly (I:C)], CgMyD88-1 and CgMyD88-2 show differences in their response: CgMyD88-1 accumulated as large spots in the cytoplasm, while CgMyD88-2 assembled in the cytoplasm and in the membrane. Our results support the theory that expansion of these immune genes is associated with functional diversity.