Functional relationship between CgMyD88-1 and CgMyD88-2 in the Pacific oyster

MyD88 is a universal adapter protein for the Toll-like receptor/interleukin-1 receptor (TLR/IL-1R) signaling pathway. Since invertebrates are believed to lack MyD88-independent pathways, MyD88 appears more critical in oyster TLR signaling pathway. In the Pacific oyster (Crassostrea gigas), two compl...

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Bibliographic Details
Published in:Fish & Shellfish Immunology
Main Authors: Tang, Xueying, Huang, Baoyu, Lin, Siheng, Wang, Wei, Zhang, Guofan, Li, Li
Format: Report
Language:English
Published: ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD 2020
Subjects:
Crassostrea gigas
Innate immunity
MyD88
Functional diversity
Fisheries
Immunology
Marine & Freshwater Biology
Veterinary Sciences
FACTOR 88 MYD88
IDENTIFICATION
GENOME
Pacific
Pacific oyster
Online Access:http://ir.qdio.ac.cn/handle/337002/164483
https://doi.org/10.1016/j.fsi.2019.10.060
Description
Summary:MyD88 is a universal adapter protein for the Toll-like receptor/interleukin-1 receptor (TLR/IL-1R) signaling pathway. Since invertebrates are believed to lack MyD88-independent pathways, MyD88 appears more critical in oyster TLR signaling pathway. In the Pacific oyster (Crassostrea gigas), two complete paralogues, named as CgMyD88-1 and CgMyD88-2, have been identified. In the current study, we indicated that CgMyD88-1 and CgMyD88-2 might act synergistically to increase the efficiency of immune signaling by activating NF-kappa B transcription factor. However, we found that upon stimulation with lipopolysaccharide (LPS) or polyinosinic:polycytidylic acid [poly (I:C)], CgMyD88-1 and CgMyD88-2 show differences in their response: CgMyD88-1 accumulated as large spots in the cytoplasm, while CgMyD88-2 assembled in the cytoplasm and in the membrane. Our results support the theory that expansion of these immune genes is associated with functional diversity.

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