The Molecular Differentiation of Anatomically Paired Left and Right Mantles of the Pacific Oyster Crassostrea gigas
Left-right (L-R) asymmetry is controlled by gene regulation pathways for the L-R axis, and in vertebrates, the gene Pitx2 in TGF-beta signaling pathway plays important roles in the asymmetrical formation of organs. However, less is known about the asymmetries of anatomically identical paired organs,...
Published in: | Marine Biotechnology |
---|---|
Main Authors: | , , , , , , , , , |
Format: | Report |
Language: | English |
Published: |
SPRINGER
2018
|
Subjects: | |
Online Access: | http://ir.qdio.ac.cn/handle/337002/163482 https://doi.org/10.1007/s10126-018-9806-8 |
id |
ftchinacasciocas:oai:ir.qdio.ac.cn:337002/163482 |
---|---|
record_format |
openpolar |
spelling |
ftchinacasciocas:oai:ir.qdio.ac.cn:337002/163482 2023-05-15T15:58:33+02:00 The Molecular Differentiation of Anatomically Paired Left and Right Mantles of the Pacific Oyster Crassostrea gigas Wei, Lei Xu, Fei Wang, Yuzhi Cai, Zhongqiang Yu, Wenchao He, Cheng Jiang, Qiuyun Xu, Xiqiang Guo, Wen Wang, Xiaotong 2018-08-01 http://ir.qdio.ac.cn/handle/337002/163482 https://doi.org/10.1007/s10126-018-9806-8 英语 eng SPRINGER MARINE BIOTECHNOLOGY http://ir.qdio.ac.cn/handle/337002/163482 doi:10.1007/s10126-018-9806-8 Pacific oyster Asymmetry of paired organs Mantle The gene Pitx Methylation Promoter region Biotechnology & Applied Microbiology Marine & Freshwater Biology LEFT-RIGHT ASYMMETRY GENE-EXPRESSION DNA METHYLATION EVOLUTION PATHWAY PITX2 VERTEBRATES ALIGNMENT BILATERIA PROTEIN 期刊论文 2018 ftchinacasciocas https://doi.org/10.1007/s10126-018-9806-8 2022-06-27T05:41:24Z Left-right (L-R) asymmetry is controlled by gene regulation pathways for the L-R axis, and in vertebrates, the gene Pitx2 in TGF-beta signaling pathway plays important roles in the asymmetrical formation of organs. However, less is known about the asymmetries of anatomically identical paired organs, as well as the transcriptional regulation mechanism of the gene Pitx in invertebrates. Here, we report the molecular biological differences between the left and right mantles of an invertebrate, the Pacific oyster Crassostrea gigas, and propose one possible mechanism underlying those differences. RNA sequencing (RNA-seq) analysis indicated that the paired organs showed different gene expression patterns, suggesting possible functional differences in shell formation, pheromone signaling, nerve conduction, the stress response, and other physiological processes. RNA-seq and real-time qPCR analysis indicated high right-side expression of the Pitx homolog (cgPitx) in oyster mantle, supporting a conserved role for Pitx in controlling asymmetry. Methylation-dependent restriction-site associated DNA sequencing (MethylRAD) identified a methylation site in the promoter region of cgPitx and showed significantly different methylation levels between the left and right mantles. This is the first report, to our knowledge, of such a difference in methylation in spiralians, and it was further confirmed in 18 other individuals by using a pyrosequencing assay. The miRNome analysis and the TGF-beta receptor/Smad inhibition experiment further supported that several genes in TGF-beta signaling pathway may be related with the L/R asymmetry of oyster mantles. These results suggested that the molecular differentiation of the oyster's paired left and right mantles is significant, TGF-beta signaling pathway could be involved in establishing or maintaining the asymmetry, and the cgPitx gene as one of genes in this pathway; the different methylation levels in its promoter regions between L/R mantles was the one of possible mechanisms regulating ... Report Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Marine Biotechnology 20 4 425 435 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Pacific oyster Asymmetry of paired organs Mantle The gene Pitx Methylation Promoter region Biotechnology & Applied Microbiology Marine & Freshwater Biology LEFT-RIGHT ASYMMETRY GENE-EXPRESSION DNA METHYLATION EVOLUTION PATHWAY PITX2 VERTEBRATES ALIGNMENT BILATERIA PROTEIN |
spellingShingle |
Pacific oyster Asymmetry of paired organs Mantle The gene Pitx Methylation Promoter region Biotechnology & Applied Microbiology Marine & Freshwater Biology LEFT-RIGHT ASYMMETRY GENE-EXPRESSION DNA METHYLATION EVOLUTION PATHWAY PITX2 VERTEBRATES ALIGNMENT BILATERIA PROTEIN Wei, Lei Xu, Fei Wang, Yuzhi Cai, Zhongqiang Yu, Wenchao He, Cheng Jiang, Qiuyun Xu, Xiqiang Guo, Wen Wang, Xiaotong The Molecular Differentiation of Anatomically Paired Left and Right Mantles of the Pacific Oyster Crassostrea gigas |
topic_facet |
Pacific oyster Asymmetry of paired organs Mantle The gene Pitx Methylation Promoter region Biotechnology & Applied Microbiology Marine & Freshwater Biology LEFT-RIGHT ASYMMETRY GENE-EXPRESSION DNA METHYLATION EVOLUTION PATHWAY PITX2 VERTEBRATES ALIGNMENT BILATERIA PROTEIN |
description |
Left-right (L-R) asymmetry is controlled by gene regulation pathways for the L-R axis, and in vertebrates, the gene Pitx2 in TGF-beta signaling pathway plays important roles in the asymmetrical formation of organs. However, less is known about the asymmetries of anatomically identical paired organs, as well as the transcriptional regulation mechanism of the gene Pitx in invertebrates. Here, we report the molecular biological differences between the left and right mantles of an invertebrate, the Pacific oyster Crassostrea gigas, and propose one possible mechanism underlying those differences. RNA sequencing (RNA-seq) analysis indicated that the paired organs showed different gene expression patterns, suggesting possible functional differences in shell formation, pheromone signaling, nerve conduction, the stress response, and other physiological processes. RNA-seq and real-time qPCR analysis indicated high right-side expression of the Pitx homolog (cgPitx) in oyster mantle, supporting a conserved role for Pitx in controlling asymmetry. Methylation-dependent restriction-site associated DNA sequencing (MethylRAD) identified a methylation site in the promoter region of cgPitx and showed significantly different methylation levels between the left and right mantles. This is the first report, to our knowledge, of such a difference in methylation in spiralians, and it was further confirmed in 18 other individuals by using a pyrosequencing assay. The miRNome analysis and the TGF-beta receptor/Smad inhibition experiment further supported that several genes in TGF-beta signaling pathway may be related with the L/R asymmetry of oyster mantles. These results suggested that the molecular differentiation of the oyster's paired left and right mantles is significant, TGF-beta signaling pathway could be involved in establishing or maintaining the asymmetry, and the cgPitx gene as one of genes in this pathway; the different methylation levels in its promoter regions between L/R mantles was the one of possible mechanisms regulating ... |
format |
Report |
author |
Wei, Lei Xu, Fei Wang, Yuzhi Cai, Zhongqiang Yu, Wenchao He, Cheng Jiang, Qiuyun Xu, Xiqiang Guo, Wen Wang, Xiaotong |
author_facet |
Wei, Lei Xu, Fei Wang, Yuzhi Cai, Zhongqiang Yu, Wenchao He, Cheng Jiang, Qiuyun Xu, Xiqiang Guo, Wen Wang, Xiaotong |
author_sort |
Wei, Lei |
title |
The Molecular Differentiation of Anatomically Paired Left and Right Mantles of the Pacific Oyster Crassostrea gigas |
title_short |
The Molecular Differentiation of Anatomically Paired Left and Right Mantles of the Pacific Oyster Crassostrea gigas |
title_full |
The Molecular Differentiation of Anatomically Paired Left and Right Mantles of the Pacific Oyster Crassostrea gigas |
title_fullStr |
The Molecular Differentiation of Anatomically Paired Left and Right Mantles of the Pacific Oyster Crassostrea gigas |
title_full_unstemmed |
The Molecular Differentiation of Anatomically Paired Left and Right Mantles of the Pacific Oyster Crassostrea gigas |
title_sort |
molecular differentiation of anatomically paired left and right mantles of the pacific oyster crassostrea gigas |
publisher |
SPRINGER |
publishDate |
2018 |
url |
http://ir.qdio.ac.cn/handle/337002/163482 https://doi.org/10.1007/s10126-018-9806-8 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
MARINE BIOTECHNOLOGY http://ir.qdio.ac.cn/handle/337002/163482 doi:10.1007/s10126-018-9806-8 |
op_doi |
https://doi.org/10.1007/s10126-018-9806-8 |
container_title |
Marine Biotechnology |
container_volume |
20 |
container_issue |
4 |
container_start_page |
425 |
op_container_end_page |
435 |
_version_ |
1766394315579326464 |