T-box transcription factor eomesodermin/Tbr2 in Atlantic cod (Gadus morhua L.): Molecular characterization, promoter structure and function analysis

Eomesodermin (Eomes) is a member of T-box transcription factor family and plays an important role in the regulation of a wide variety of developmental processes and immune response in animals. Here we report cloning and characterization of the full-length cDNA of Atlantic cod Eomes (GmEomes), which...

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Bibliographic Details
Published in:Fish & Shellfish Immunology
Main Authors: Chi, Heng, Sormo, Kristian Gillebo, Diao, Jing, Dalmo, Roy Ambli
Format: Report
Language:English
Published: ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD 2019
Subjects:
Eomesodermin
Atlantic cod
Molecular cloning
Promoter structure
Function analysis
Fisheries
Immunology
Marine & Freshwater Biology
Veterinary Sciences
VIBRIO-ANGUILLARUM
GENE EOMESODERMIN
RADIAL GLIA
EXPRESSION
MOUSE
BET
TISSUES
CELLS
TBR2
ACTIVATION
atlantic cod
Gadus morhua
Online Access:http://ir.qdio.ac.cn/handle/337002/163409
https://doi.org/10.1016/j.fsi.2019.07.021
Description
Summary:Eomesodermin (Eomes) is a member of T-box transcription factor family and plays an important role in the regulation of a wide variety of developmental processes and immune response in animals. Here we report cloning and characterization of the full-length cDNA of Atlantic cod Eomes (GmEomes), which possesses a TBOX_3 domain similar to its counterpart in mammals. The regulated expression was observed in head kidney and spleen in response to live Vibrio anguillarum infection in vivo, and spleen leukocytes in vitro after PMA and poly I:C stimulation. Furthermore, we determined a 694 bp sequence, upstream of the transcriptional start site (TSS), to contain a number of sequence motifs that matched known transcription factor-binding sites, Activities of the presumptive regulatory gene were assessed by transfecting different 5'-deletion constructs in CHSE-214 cells. The results showed that the basal promoters and positive transcriptional regulator activities of GmEomes were dependent by sequences located from -694 to -376 bp upstream of TSS. Furthermore, we found that some Eomes binding sites were present in the 5'-flanking regions of the cod IFN gamma gene predicted by bioinformatics. However, Co-transfection of eomesodermin overexpression plasmids with INF gamma reporter vector into CHSE-214 cells determined that Atlantic cod eomesodermin played a minor role in activation of the INF gamma promoter.

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