Network of microRNA-transcriptional factor-mRNA in cold response of turbot Scophthalmus maximus

The aim of this study is to understand fish cold-tolerant mechanism. We analyzed the transcriptional reactions to the cold condition in turbot Scophthalmus maximus by using RNA-seq and microRNA (miRNA)-seq. Meio-gynogenetic diploid turbots were treated at 0 degrees C to distinguish the cold-tolerant...

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Bibliographic Details
Published in:Fish Physiology and Biochemistry
Main Authors: Nie, Miaomiao, Tan, Xungang, Lu, Yunliang, Wu, Zhihao, Li, Jun, Xu, Dongdong, Zhang, Peijun, You, Feng
Format: Report
Language:English
Published: SPRINGER 2019
Subjects:
Scophthalmus maximus
Cold tolerance
Transcription
microRNA
Transcription factor
Biochemistry & Molecular Biology
Fisheries
Physiology
FLOUNDER-PARALICHTHYS-OLIVACEUS
OXIDATIVE STRESS
GENE-REGULATION
CELL CARCINOMA
EXPRESSION
ZEBRAFISH
EVOLUTION
TEMPERATURE
TISSUES
MIR-217
Turbot
Online Access:http://ir.qdio.ac.cn/handle/337002/161609
https://doi.org/10.1007/s10695-019-00611-y
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Summary:The aim of this study is to understand fish cold-tolerant mechanism. We analyzed the transcriptional reactions to the cold condition in turbot Scophthalmus maximus by using RNA-seq and microRNA (miRNA)-seq. Meio-gynogenetic diploid turbots were treated at 0 degrees C to distinguish the cold-tolerant (CT) and cold-sensitive (CS) groups. The results showed that there were quite different responses at both mRNA and miRNA levels, with more up-regulated mRNAs (1069 vs. 194) and less down-regulated miRNAs (4 vs. 1) in CT versus CS relative to the control group. The network of miRNA-transcription factor-mRNA, regulating turbot different response to cold stress, was constructed, which involved in cell cycle, component of cell membrane, signal transduction, and circadian rhythm pathways. The above information demonstrates mechanisms by which cold tolerance is increased in fish.

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