High-Throughput Identification of Antimicrobial Peptides from Amphibious Mudskippers
Widespread existence of antimicrobial peptides (AMPs) has been reported in various animals with comprehensive biological activities, which is consistent with the important roles of AMPs as the first line of host defense system. However, no big-data-based analysis on AMPs from any fish species is ava...
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MDPI AG
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Online Access: | http://ir.qdio.ac.cn/handle/337002/157298 https://doi.org/10.3390/md15110364 |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/157298 2023-05-15T15:58:44+02:00 High-Throughput Identification of Antimicrobial Peptides from Amphibious Mudskippers Yi, Yunhai You, Xinxin Bian, Chao Chen, Shixi Lv, Zhao Qiu, Limei Shi, Qiong 2017-11-01 http://ir.qdio.ac.cn/handle/337002/157298 https://doi.org/10.3390/md15110364 英语 eng MDPI AG MARINE DRUGS http://ir.qdio.ac.cn/handle/337002/157298 doi:10.3390/md15110364 antimicrobial peptide (AMP) high-throughput identification amphibious mudskipper antimicrobial activity Pharmacology & Pharmacy Chemistry Medicinal HOST-DEFENSE PEPTIDES ANTIBACTERIAL PEPTIDES FUNCTIONAL-ANALYSIS CRASSOSTREA-GIGAS PACIFIC OYSTER 2 LEAP-2 HEMOGLOBIN MECHANISM FISH UBIQUICIDIN 期刊论文 2017 ftchinacasciocas https://doi.org/10.3390/md15110364 2022-06-27T05:39:24Z Widespread existence of antimicrobial peptides (AMPs) has been reported in various animals with comprehensive biological activities, which is consistent with the important roles of AMPs as the first line of host defense system. However, no big-data-based analysis on AMPs from any fish species is available. In this study, we identified 507 AMP transcripts on the basis of our previously reported genomes and transcriptomes of two representative amphibious mudskippers, Boleophthalmus pectinirostris (BP) and Periophthalmus magnuspinnatus (PM). The former is predominantly aquatic with less time out of water, while the latter is primarily terrestrial with extended periods of time on land. Within these identified AMPs, 449 sequences are novel; 15 were reported in BP previously; 48 are identically overlapped between BP and PM; 94 were validated by mass spectrometry. Moreover, most AMPs presented differential tissue transcription patterns in the two mudskippers. Interestingly, we discovered two AMPs, hemoglobin 1 and amylin, with high inhibitions on Micrococcus luteus. In conclusion, our high-throughput screening strategy based on genomic and transcriptomic data opens an efficient pathway to discover new antimicrobial peptides for ongoing development of marine drugs. Report Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Marine Drugs 15 11 364 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
antimicrobial peptide (AMP) high-throughput identification amphibious mudskipper antimicrobial activity Pharmacology & Pharmacy Chemistry Medicinal HOST-DEFENSE PEPTIDES ANTIBACTERIAL PEPTIDES FUNCTIONAL-ANALYSIS CRASSOSTREA-GIGAS PACIFIC OYSTER 2 LEAP-2 HEMOGLOBIN MECHANISM FISH UBIQUICIDIN |
spellingShingle |
antimicrobial peptide (AMP) high-throughput identification amphibious mudskipper antimicrobial activity Pharmacology & Pharmacy Chemistry Medicinal HOST-DEFENSE PEPTIDES ANTIBACTERIAL PEPTIDES FUNCTIONAL-ANALYSIS CRASSOSTREA-GIGAS PACIFIC OYSTER 2 LEAP-2 HEMOGLOBIN MECHANISM FISH UBIQUICIDIN Yi, Yunhai You, Xinxin Bian, Chao Chen, Shixi Lv, Zhao Qiu, Limei Shi, Qiong High-Throughput Identification of Antimicrobial Peptides from Amphibious Mudskippers |
topic_facet |
antimicrobial peptide (AMP) high-throughput identification amphibious mudskipper antimicrobial activity Pharmacology & Pharmacy Chemistry Medicinal HOST-DEFENSE PEPTIDES ANTIBACTERIAL PEPTIDES FUNCTIONAL-ANALYSIS CRASSOSTREA-GIGAS PACIFIC OYSTER 2 LEAP-2 HEMOGLOBIN MECHANISM FISH UBIQUICIDIN |
description |
Widespread existence of antimicrobial peptides (AMPs) has been reported in various animals with comprehensive biological activities, which is consistent with the important roles of AMPs as the first line of host defense system. However, no big-data-based analysis on AMPs from any fish species is available. In this study, we identified 507 AMP transcripts on the basis of our previously reported genomes and transcriptomes of two representative amphibious mudskippers, Boleophthalmus pectinirostris (BP) and Periophthalmus magnuspinnatus (PM). The former is predominantly aquatic with less time out of water, while the latter is primarily terrestrial with extended periods of time on land. Within these identified AMPs, 449 sequences are novel; 15 were reported in BP previously; 48 are identically overlapped between BP and PM; 94 were validated by mass spectrometry. Moreover, most AMPs presented differential tissue transcription patterns in the two mudskippers. Interestingly, we discovered two AMPs, hemoglobin 1 and amylin, with high inhibitions on Micrococcus luteus. In conclusion, our high-throughput screening strategy based on genomic and transcriptomic data opens an efficient pathway to discover new antimicrobial peptides for ongoing development of marine drugs. |
format |
Report |
author |
Yi, Yunhai You, Xinxin Bian, Chao Chen, Shixi Lv, Zhao Qiu, Limei Shi, Qiong |
author_facet |
Yi, Yunhai You, Xinxin Bian, Chao Chen, Shixi Lv, Zhao Qiu, Limei Shi, Qiong |
author_sort |
Yi, Yunhai |
title |
High-Throughput Identification of Antimicrobial Peptides from Amphibious Mudskippers |
title_short |
High-Throughput Identification of Antimicrobial Peptides from Amphibious Mudskippers |
title_full |
High-Throughput Identification of Antimicrobial Peptides from Amphibious Mudskippers |
title_fullStr |
High-Throughput Identification of Antimicrobial Peptides from Amphibious Mudskippers |
title_full_unstemmed |
High-Throughput Identification of Antimicrobial Peptides from Amphibious Mudskippers |
title_sort |
high-throughput identification of antimicrobial peptides from amphibious mudskippers |
publisher |
MDPI AG |
publishDate |
2017 |
url |
http://ir.qdio.ac.cn/handle/337002/157298 https://doi.org/10.3390/md15110364 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
MARINE DRUGS http://ir.qdio.ac.cn/handle/337002/157298 doi:10.3390/md15110364 |
op_doi |
https://doi.org/10.3390/md15110364 |
container_title |
Marine Drugs |
container_volume |
15 |
container_issue |
11 |
container_start_page |
364 |
_version_ |
1766394508778405888 |