High-Throughput Identification of Antimicrobial Peptides from Amphibious Mudskippers

Widespread existence of antimicrobial peptides (AMPs) has been reported in various animals with comprehensive biological activities, which is consistent with the important roles of AMPs as the first line of host defense system. However, no big-data-based analysis on AMPs from any fish species is ava...

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Bibliographic Details
Published in:Marine Drugs
Main Authors: Yi, Yunhai, You, Xinxin, Bian, Chao, Chen, Shixi, Lv, Zhao, Qiu, Limei, Shi, Qiong
Format: Report
Language:English
Published: MDPI AG 2017
Subjects:
antimicrobial peptide (AMP)
high-throughput identification
amphibious mudskipper
antimicrobial activity
Pharmacology & Pharmacy
Chemistry
Medicinal
HOST-DEFENSE PEPTIDES
ANTIBACTERIAL PEPTIDES
FUNCTIONAL-ANALYSIS
CRASSOSTREA-GIGAS
PACIFIC OYSTER
2 LEAP-2
HEMOGLOBIN
MECHANISM
FISH
UBIQUICIDIN
Pacific
Crassostrea gigas
Pacific oyster
Online Access:http://ir.qdio.ac.cn/handle/337002/157298
https://doi.org/10.3390/md15110364
Description
Summary:Widespread existence of antimicrobial peptides (AMPs) has been reported in various animals with comprehensive biological activities, which is consistent with the important roles of AMPs as the first line of host defense system. However, no big-data-based analysis on AMPs from any fish species is available. In this study, we identified 507 AMP transcripts on the basis of our previously reported genomes and transcriptomes of two representative amphibious mudskippers, Boleophthalmus pectinirostris (BP) and Periophthalmus magnuspinnatus (PM). The former is predominantly aquatic with less time out of water, while the latter is primarily terrestrial with extended periods of time on land. Within these identified AMPs, 449 sequences are novel; 15 were reported in BP previously; 48 are identically overlapped between BP and PM; 94 were validated by mass spectrometry. Moreover, most AMPs presented differential tissue transcription patterns in the two mudskippers. Interestingly, we discovered two AMPs, hemoglobin 1 and amylin, with high inhibitions on Micrococcus luteus. In conclusion, our high-throughput screening strategy based on genomic and transcriptomic data opens an efficient pathway to discover new antimicrobial peptides for ongoing development of marine drugs.

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