Involvement of clustered oyster Wnt genes in gut formation

Genes encoding Wnt ligands play important roles in organ development. The Wnt10-Wnt6-Wnt1-Wnt9 cluster widely presents in many metazoan genomes, indicating the importance of gene arrangement. Hypothesis has been proposed that they may be coordinately regulated. However, few expression correlations w...

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Bibliographic Details
Published in:Journal of Oceanology and Limnology
Main Authors: Liu Jun, Xu Fei, Ji Peng, Li Li, Zhang Guofan
Format: Report
Language:English
Published: SCIENCE PRESS 2018
Subjects:
gene cluster
tissue distribution
digestive gland
immunofluorescence
Crassostrea gigas
Marine & Freshwater Biology
Oceanography
Limnology
MYTILUS-TROSSULUS MOLLUSCA
INTESTINAL REGENERATION
EXPRESSION PATTERNS
COMPONENTS
MORPHOGENESIS
ADAPTATION
COMPLEXITY
ENDODERM
MESODERM
BIVALVIA
Online Access:http://ir.qdio.ac.cn/handle/337002/156411
https://doi.org/10.1007/s00343-018-7138-1
Description
Summary:Genes encoding Wnt ligands play important roles in organ development. The Wnt10-Wnt6-Wnt1-Wnt9 cluster widely presents in many metazoan genomes, indicating the importance of gene arrangement. Hypothesis has been proposed that they may be coordinately regulated. However, few expression correlations were identified in model animals. We analyzed the tissue expression pattern of clustered oyster Wnt10, Wnt6, Wnt1, and Wnt9a genes in this study. The results indicated the highest expression level in adult gut system of these clustered Wnt genes, except for Wnt6, which had highest expression in mantle. Further whole-mount immunofluorescence assay indicated that Wnt6 protein was restricted to gut region in oyster larvae. These results suggest the possible important role of the Wnt10-Wnt6-Wnt1-Wnt9 cluster in oyster gut formation.

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