CgA1AR-1 acts as an alpha-1 adrenergic receptor in oyster Crassostrea gigas mediating both cellular and humoral immune response

We have now cloned an alpha-1 adrenergic receptor (A1AR) from the cDNA library of oyster Crassostrea gigas, designating as CgA1AR-1. The full length of CgA1AR-1 was 1149 bp and it encodes a protein of 382 amino acids containing a 7 transmembrane domain, whose putative topology was similar to the A1A...

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Bibliographic Details
Published in:Fish & Shellfish Immunology
Main Authors: Liu, Zhaoqun, Zhou, Zhi, Wang, Lingling, Qiu, Limei, Zhang, Huan, Wang, Hao, Song, Linsheng
Format: Article in Journal/Newspaper
Language:English
Published: 2016
Subjects:
Crassostrea Gigas
Norepinephrine
Alpha-1 Adrenergic Receptor
Tnf Pathway
Immune Regulation
Crassostrea gigas
Online Access:http://ir.qdio.ac.cn/handle/337002/136200
https://doi.org/10.1016/j.fsi.2016.09.022
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Summary:We have now cloned an alpha-1 adrenergic receptor (A1AR) from the cDNA library of oyster Crassostrea gigas, designating as CgA1AR-1. The full length of CgA1AR-1 was 1149 bp and it encodes a protein of 382 amino acids containing a 7 transmembrane domain, whose putative topology was similar to the A1ARs in higher organisms and shared similarity of 19% with mammalian A1ARs according to the phylogenic analysis. After cell transfection of CgA1AR-1 into HEK293T cells and the incubation with its specific agonist norepinephrine (NE), the concentration of second messenger Ca2+ increased significantly (p < 0.05). But, this increasing of Ca2+ could be inhibited by adding AJAR antagonist DOX. Tissue distribution assays using qRT-PCR suggested that CgA1AR-1 mRNA was ubiquitously expressed in all the major tissues of oyster. LPS stimulation could induce the up-regulation of CgA1AR-1 mRNA in haemocytes from 12 h to 24 h post stimulation. Moreover, the blocking of CgA1AR-1 by DOX before LPS stimulation affected the mRNA expression of oyster TNF (CGI_10005109 and CGL_10006440) in haemocytes, resulting in the rise of haemocyte phagocytic rate and apoptosis index. In addition to cellular immunity, CgA1AR-1 was also involved in humoral immunity of oyster. Inhibition of CgA1AR-1 with DOX could repress the up-regulation of LZY and SOD activities caused by LPS stimulation. These results suggested that CgA1AR-1 acted as an alpha-1 adrenergic receptor in cetacholaminergic neuroendocrineimmune network mediating both cellular and humoral immune response. (C) 2016 Elsevier Ltd. All rights reserved.

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