The inhibitory role of gamma-aminobutyric acid (GABA) on immunomodulation of Pacific oyster Crassostrea gigas
gamma-aminobutyric acid (GABA) is an inhibitory neurotransmitter to suppress the immune-mediated pro inflammatory reactions, and it has been used in the treatment of many inflammation-related diseases in vertebrates, while its immunomodulatory role in invertebrates has never been reported. In the pr...
Published in: | Fish & Shellfish Immunology |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/131034 2023-05-15T15:57:42+02:00 The inhibitory role of gamma-aminobutyric acid (GABA) on immunomodulation of Pacific oyster Crassostrea gigas Li, Meijia Qiu, Limei Wang, Lingling Wang, Weilin Xin, Lusheng Li, Yiqun Liu, Zhaoqun Song, Linsheng 2016-05-01 http://ir.qdio.ac.cn/handle/337002/131034 https://doi.org/10.1016/j.fsi.2016.03.015 英语 eng FISH & SHELLFISH IMMUNOLOGY Li, Meijia,Qiu, Limei,Wang, Lingling,et al. The inhibitory role of gamma-aminobutyric acid (GABA) on immunomodulation of Pacific oyster Crassostrea gigas[J]. FISH & SHELLFISH IMMUNOLOGY,2016,52:16-22. http://ir.qdio.ac.cn/handle/337002/131034 doi:10.1016/j.fsi.2016.03.015 Crassostrea Gigas Inhibitory Neurotransmitter Gamma-aminobutyric Acid Immunomodulation Immune Response Article 期刊论文 2016 ftchinacasciocas https://doi.org/10.1016/j.fsi.2016.03.015 2022-06-27T05:37:39Z gamma-aminobutyric acid (GABA) is an inhibitory neurotransmitter to suppress the immune-mediated pro inflammatory reactions, and it has been used in the treatment of many inflammation-related diseases in vertebrates, while its immunomodulatory role in invertebrates has never been reported. In the present study, GABA was found to exist in the hemolymph of Pacific oyster Crassostrea gigas, and its concentration decreased slightly from 8.00 +/- 0.37 mu mol L-1 at normal condition to 7.73 +/- 0.15 mu mol L-1 at 6 h after LPS stimulation, and then increased to 9.34 +/- 0.15 mu mol L-1, 8.86 +/- 0.68 mu mol L-1 at 12 h and 48 h, respectively. After LPS stimulation, the mRNA expressions of pro-inflammatory cytokines (CgIL-17 and CgTNF) and immune effectors (CgSOD and CgBPI), and the protein expression of NOS increased significantly, and these increased trends were remarkably inhibited by GABA stimulation. At the same time, the phagocytosis rate and apoptosis rate of immunocytes also increased obviously after LPS stimulation, whereas the increase was repressed with the addition of GABA. The results collectively demonstrated that GABA was an indispensable inhibitory agent for both humoral and cellular immune response, which mainly functioned at the late phase of immune response to avoid the excess immune reactions and maintain the immune homeostasis. (C) 2016 Elsevier Ltd. All rights reserved. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Fish & Shellfish Immunology 52 16 22 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Crassostrea Gigas Inhibitory Neurotransmitter Gamma-aminobutyric Acid Immunomodulation Immune Response |
spellingShingle |
Crassostrea Gigas Inhibitory Neurotransmitter Gamma-aminobutyric Acid Immunomodulation Immune Response Li, Meijia Qiu, Limei Wang, Lingling Wang, Weilin Xin, Lusheng Li, Yiqun Liu, Zhaoqun Song, Linsheng The inhibitory role of gamma-aminobutyric acid (GABA) on immunomodulation of Pacific oyster Crassostrea gigas |
topic_facet |
Crassostrea Gigas Inhibitory Neurotransmitter Gamma-aminobutyric Acid Immunomodulation Immune Response |
description |
gamma-aminobutyric acid (GABA) is an inhibitory neurotransmitter to suppress the immune-mediated pro inflammatory reactions, and it has been used in the treatment of many inflammation-related diseases in vertebrates, while its immunomodulatory role in invertebrates has never been reported. In the present study, GABA was found to exist in the hemolymph of Pacific oyster Crassostrea gigas, and its concentration decreased slightly from 8.00 +/- 0.37 mu mol L-1 at normal condition to 7.73 +/- 0.15 mu mol L-1 at 6 h after LPS stimulation, and then increased to 9.34 +/- 0.15 mu mol L-1, 8.86 +/- 0.68 mu mol L-1 at 12 h and 48 h, respectively. After LPS stimulation, the mRNA expressions of pro-inflammatory cytokines (CgIL-17 and CgTNF) and immune effectors (CgSOD and CgBPI), and the protein expression of NOS increased significantly, and these increased trends were remarkably inhibited by GABA stimulation. At the same time, the phagocytosis rate and apoptosis rate of immunocytes also increased obviously after LPS stimulation, whereas the increase was repressed with the addition of GABA. The results collectively demonstrated that GABA was an indispensable inhibitory agent for both humoral and cellular immune response, which mainly functioned at the late phase of immune response to avoid the excess immune reactions and maintain the immune homeostasis. (C) 2016 Elsevier Ltd. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Li, Meijia Qiu, Limei Wang, Lingling Wang, Weilin Xin, Lusheng Li, Yiqun Liu, Zhaoqun Song, Linsheng |
author_facet |
Li, Meijia Qiu, Limei Wang, Lingling Wang, Weilin Xin, Lusheng Li, Yiqun Liu, Zhaoqun Song, Linsheng |
author_sort |
Li, Meijia |
title |
The inhibitory role of gamma-aminobutyric acid (GABA) on immunomodulation of Pacific oyster Crassostrea gigas |
title_short |
The inhibitory role of gamma-aminobutyric acid (GABA) on immunomodulation of Pacific oyster Crassostrea gigas |
title_full |
The inhibitory role of gamma-aminobutyric acid (GABA) on immunomodulation of Pacific oyster Crassostrea gigas |
title_fullStr |
The inhibitory role of gamma-aminobutyric acid (GABA) on immunomodulation of Pacific oyster Crassostrea gigas |
title_full_unstemmed |
The inhibitory role of gamma-aminobutyric acid (GABA) on immunomodulation of Pacific oyster Crassostrea gigas |
title_sort |
inhibitory role of gamma-aminobutyric acid (gaba) on immunomodulation of pacific oyster crassostrea gigas |
publishDate |
2016 |
url |
http://ir.qdio.ac.cn/handle/337002/131034 https://doi.org/10.1016/j.fsi.2016.03.015 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
FISH & SHELLFISH IMMUNOLOGY Li, Meijia,Qiu, Limei,Wang, Lingling,et al. The inhibitory role of gamma-aminobutyric acid (GABA) on immunomodulation of Pacific oyster Crassostrea gigas[J]. FISH & SHELLFISH IMMUNOLOGY,2016,52:16-22. http://ir.qdio.ac.cn/handle/337002/131034 doi:10.1016/j.fsi.2016.03.015 |
op_doi |
https://doi.org/10.1016/j.fsi.2016.03.015 |
container_title |
Fish & Shellfish Immunology |
container_volume |
52 |
container_start_page |
16 |
op_container_end_page |
22 |
_version_ |
1766393373024845824 |