The inhibitory role of gamma-aminobutyric acid (GABA) on immunomodulation of Pacific oyster Crassostrea gigas

gamma-aminobutyric acid (GABA) is an inhibitory neurotransmitter to suppress the immune-mediated pro inflammatory reactions, and it has been used in the treatment of many inflammation-related diseases in vertebrates, while its immunomodulatory role in invertebrates has never been reported. In the pr...

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Bibliographic Details
Published in:Fish & Shellfish Immunology
Main Authors: Li, Meijia, Qiu, Limei, Wang, Lingling, Wang, Weilin, Xin, Lusheng, Li, Yiqun, Liu, Zhaoqun, Song, Linsheng
Format: Article in Journal/Newspaper
Language:English
Published: 2016
Subjects:
Crassostrea Gigas
Inhibitory Neurotransmitter
Gamma-aminobutyric Acid
Immunomodulation
Immune Response
Pacific
Crassostrea gigas
Pacific oyster
Online Access:http://ir.qdio.ac.cn/handle/337002/131034
https://doi.org/10.1016/j.fsi.2016.03.015
Description
Summary:gamma-aminobutyric acid (GABA) is an inhibitory neurotransmitter to suppress the immune-mediated pro inflammatory reactions, and it has been used in the treatment of many inflammation-related diseases in vertebrates, while its immunomodulatory role in invertebrates has never been reported. In the present study, GABA was found to exist in the hemolymph of Pacific oyster Crassostrea gigas, and its concentration decreased slightly from 8.00 +/- 0.37 mu mol L-1 at normal condition to 7.73 +/- 0.15 mu mol L-1 at 6 h after LPS stimulation, and then increased to 9.34 +/- 0.15 mu mol L-1, 8.86 +/- 0.68 mu mol L-1 at 12 h and 48 h, respectively. After LPS stimulation, the mRNA expressions of pro-inflammatory cytokines (CgIL-17 and CgTNF) and immune effectors (CgSOD and CgBPI), and the protein expression of NOS increased significantly, and these increased trends were remarkably inhibited by GABA stimulation. At the same time, the phagocytosis rate and apoptosis rate of immunocytes also increased obviously after LPS stimulation, whereas the increase was repressed with the addition of GABA. The results collectively demonstrated that GABA was an indispensable inhibitory agent for both humoral and cellular immune response, which mainly functioned at the late phase of immune response to avoid the excess immune reactions and maintain the immune homeostasis. (C) 2016 Elsevier Ltd. All rights reserved.

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