Glaciimonas frigoris sp nov., a psychrophilic bacterium isolated from ancient Siberian permafrost sediment, and emended description of the genus Glaciimonas

• Published in: International Journal of Systematic and Evolutionary Microbiology
• Main Authors: Margesin, Rosa, Zhang, De-Chao, Frasson, David, Brouchkov, Anatoli
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2016
• Subjects: permafrost
• Online Access: http://ir.qdio.ac.cn/handle/337002/126247; https://doi.org/10.1099/ijsem.0.000783

The bacterial strain N1-38(T) was isolated from ancient Siberian permafrost sediment. The strain was Gram-reaction-negative, motile by gliding, rod-shaped and psychrophilic, and showed good growth over a temperature range of -5 to 25 degrees C. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain N1-38(T) was most closely related to members of the genus Glaciimonas and shared the highest 16S rRNA gene sequence similarities with the type strains of Glaciimonas alpina (99.3 %), Glaciimonas immobilis (98.9 %) and Glaciimonas singularis (96.5 %). The predominant cellular fatty acids of strain N1-38(T) were summed feature 3 (C-16 : 1 omega 7c and/or iso-C-15 : 0 2-OH), C-16 : 0 and C-18 : 1 omega 7c. The major respiratory quinone was ubiquinone 8 and the major polar lipids were phosphatidylethanolamine and diphosphatidylglycerol. The genomic DNA G+C content was 53.0 mol%. Combined data of phenotypic, phylogenetic and DNA-DNA relatedness studies demonstrated that strain N1-38(T) represents a novel species of the genus Glaciimonas, for which the name Glaciimonas frigoris sp. nov. is proposed. The type strain is N1-38(T) (=LMG 28868(T)=CCOS 838(T)). An emended description of the genus Glaciimonas is also provided.