Sexual dimorphic expression of dnd in germ cells during sex reversal and its requirement for primordial germ cell survival in protogynous hermaphroditic grouper

Dead end (dnd), vertebrate-specific germ cell marker, had been demonstrated to be essential for primordial germ cell (PGC) migration and survival, and the link between PGC number and sex change had been revealed in some teleost species, but little is known about dnd in hermaphroditic vertebrates. In...

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Bibliographic Details
Published in:Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology
Main Authors: Sun, Zhi-Hui, Zhou, Li, Li, Zhi, Liu, Xiao-Chun, Li, Shui-Sheng, Wang, Yang, Gui, Jian-Fang
Format: Report
Language:English
Published: ELSEVIER SCIENCE INC 2017
Subjects:
dnd
Morpholino
Epinephelus coioides
PGC
Sex change
Biochemistry & Molecular Biology
Zoology
DEAD-END GENE
ORANGE-SPOTTED GROUPER
EPINEPHELUS-COIOIDES
DIFFERENTIAL EXPRESSION
VERTEBRATE EMBRYOS
MOLECULAR-CLONING
ATLANTIC SALMON
MESSENGER-RNA
TER MUTATION
MARKER GENE
Atlantic salmon
Online Access:http://ir.ihb.ac.cn/handle/342005/32588
http://ir.ihb.ac.cn/handle/342005/32589
https://doi.org/10.1016/j.cbpb.2017.04.003
Description
Summary:Dead end (dnd), vertebrate-specific germ cell marker, had been demonstrated to be essential for primordial germ cell (PGC) migration and survival, and the link between PGC number and sex change had been revealed in some teleost species, but little is known about dnd in hermaphroditic vertebrates. In the present study, a protogynous hermaphroditic orange-spotted grouper (Epinephelus coioides) dnd homologue (Ecdnd) was identified and characterized. Quantitative real-time PCR and in situ hybridization analysis revealed a dynamic and sexually dimorphic expression pattern in PGCs and germ cells of gonads. During sex changing, the Ecdnd transcript sharply increased in early transitional gonad, reached the highest level at late transitional gonad stage, and decreased after testis maturation. Visualization of zebrafish PGCs by injecting with RFP-Ecdnd-3 ' UTR RNA and GFP-zfnanos3-3 ' UTR RNA confirmed importance of Ecdnd 3 ' UTR for the PGC distribution. In addition, knockdown of EcDnd by using antisense morpholinos (MO) caused the ablation of PGCs in orange-spotted grouper. Therefore, the current data indicate that Ecdnd is essential for PGCs survival and may serve as a useful germ cell marker during gametogenesis in hermaphroditic grouper.

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