Optimal dietary alpha-linolenic acid/linoleic acid ratio improved digestive and absorptive capacities and target of rapamycin gene expression of juvenile grass carp (Ctenopharyngodon idellus)

Growth performance, digestive and absorptive capacities and target of rapamycin (TOR), ribosomal protein S6 kinase 1 (S6K1) and eIF4E-binding protein (4E-BP) gene expression in the hepatopancreas and intestine of juvenile grass carp (Ctenopharyngodon idellus) fed graded ratios of dietary alpha-linol...

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Bibliographic Details
Published in:Aquaculture Nutrition
Main Authors: Zeng, Y. -Y., Jiang, W. -D., Liu, Y., Wu, P., Zhao, J., Jiang, J., Kuang, S. -Y., Tang, L., Tang, W. -N., Zhang, Y. -A., Zhou, X. -Q., Feng, L.
Format: Report
Language:English
Published: WILEY-BLACKWELL 2016
Subjects:
absorption
alpha-linolenic acid
Ctenopharyngodon idellus
digestion
linoleic acid
TOR signalling pathway
Fisheries
INTESTINAL ENZYME-ACTIVITIES
TROUT ONCORHYNCHUS-MYKISS
ESSENTIAL FATTY-ACIDS
TURBOT SCOPHTHALMUS-MAXIMUS
SEABREAM SPARUS-AURATA
SALMON SALMO-SALAR
FED GRADED-LEVELS
GROWTH-PERFORMANCE
RAINBOW-TROUT
VAR. JIAN
Salmo salar
Scophthalmus maximus
Turbot
Online Access:http://ir.ihb.ac.cn/handle/342005/31916
http://ir.ihb.ac.cn/handle/342005/31917
https://doi.org/10.1111/anu.12337
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Summary:Growth performance, digestive and absorptive capacities and target of rapamycin (TOR), ribosomal protein S6 kinase 1 (S6K1) and eIF4E-binding protein (4E-BP) gene expression in the hepatopancreas and intestine of juvenile grass carp (Ctenopharyngodon idellus) fed graded ratios of dietary alpha-linolenic acid/linoleic acid (ALA/LNA) (0.01, 0.34, 0.68, 1.03, 1.41, 1.76 and 2.15) for 60days were investigated. The results showed that ALA/LNA ratio of 1.03 significantly improved (i) per cent weight gain (PWG) and feed efficiency, (ii) hepatopancreatic trypsin, chymotrypsin, lipase, amylase and intestinal creatine kinase (CK) activities, (iii) hepatopancreatic trypsinogen-2 and chymotrypsinogen mRNA levels. Meanwhile, fish fed with ALA/LNA ratio of 0.68 significantly enhanced, (iv) Na+/K+-ATPase and -glutamyl transpeptidase activities in whole intestine, and alkaline phosphatase activities in the proximal intestine (PI) and distal intestine, (v) amylase, intestinal Na+/K+-ATPase alpha-subunit isoform 1, Na+/K+-ATPase alpha-subunit isoform 8 and CK mRNA abundances, (vi) TOR and S6K1 gene expression in the hepatopancreas and intestine of juvenile grass carp. Based on the quadratic regression analysis of PWG, cholecystokinin and leptin contents in the PI, optimal dietary ALA/LNA ratio of juvenile grass carp (8.78-72.00g) was estimated to be 1.08, 1.19 and 1.05, respectively.

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