Deinococcus psychrotolerans sp. nov., isolated from soil on the South Shetland Islands, Antarctica

A Gram-stain-negative, non-motile, strictly aerobic, coccus-shaped bacterium, designated S14-83(T), was isolated from a soil sample collected from the South Shetland Islands of Antarctica. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain is a novel member of the genus...

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Bibliographic Details
Published in:International Journal of Systematic and Evolutionary Microbiology
Main Authors: Tian, Jin, Wang, Liqiu, Liu, Ping, Geng, Yingchao, Zhu, Guoxin, Zheng, Ruichen, Liu, Zuobing, Zhao, Yiming, Yang, Jian, Peng, Fang
Format: Article in Journal/Newspaper
Language:English
Published: MICROBIOLOGY SOC 2019
Subjects:
Deinococcus psychrotolerans
Antarctica
radiation resistance
Microbiology
RADIATION-RESISTANT BACTERIUM
IONIZING-RADIATION
RADIODURANS
SEQUENCES
South Shetland Islands
Antarc*
Online Access:http://ir.ibcas.ac.cn/handle/2S10CLM1/19502
https://doi.org/10.1099/ijsem.0.003484
Description
Summary:A Gram-stain-negative, non-motile, strictly aerobic, coccus-shaped bacterium, designated S14-83(T), was isolated from a soil sample collected from the South Shetland Islands of Antarctica. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain is a novel member of the genus Deinococcus, with Deinococcus alpinitundrae as its closest relative (96.1 % similarity). The DNA G+C content of the strain was 61.1 mol% and the major respiratory quinone was MK-8. Major cellular fatty acids were summed feature 3 (C-16:1 omega 7c/C-16:1 omega 6c) and C-16:0. As well as containing glycophospholipid, aminophospholipids and glycolipid as major polar lipids, there were also some unknown polar lipids. The diagnostic diamino acid in the cell-wall peptidoglycan was ornithine, corroborating the assignment of the strain to the genus Deinococcus. Strain S14-83(T) was shown to be extremely resistant to gamma radiation (>10 kGy) and UV light (460 Jm(-2)). On the basis of phylogenetic, chemotaxonomic and phenotypic data presented here, strain 514-83(T) represents a novel species of the genus Deinococcus, for which the name Deinococcus psychrotolerans sp. nov. is proposed. The type strain is S14-83(T) (=CCTCC AB 2015449(T) = DSM 105285(T)).

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