Population genetic structure and identification of loci under selection in the invasive tunicate, Botryllus schlosseri, using newly developed EST-SSRs

Botryllus schlosseri, also known as golden star tunicate, is generally considered of European origin and has successfully invaded coastal waters of all continents except Antarctica. Owing to its significantly negative ecological impacts, it is crucial to understand its dispersal dynamics and mechani...

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Bibliographic Details
Published in:Biochemical Systematics and Ecology
Main Authors: Lin, Yaping, Zhan, Aibin
Format: Report
Language:unknown
Published: 2016
Subjects:
Biological Invasion
Botryllus Schlosseri
Genetic Diversity
Golden Star Tunicate
Microsatellite
Invasive Species
Tunicate
Antarc*
Antarctica
Online Access:http://ir.rcees.ac.cn/handle/311016/36141
https://doi.org/10.1016/j.bse.2016.05.007
Description
Summary:Botryllus schlosseri, also known as golden star tunicate, is generally considered of European origin and has successfully invaded coastal waters of all continents except Antarctica. Owing to its significantly negative ecological impacts, it is crucial to understand its dispersal dynamics and mechanisms of invasion success. Here, we identified 1020 microsatellite-containing sequences from 98,626 expressed sequence tags (ESTs), and developed and characterized 17 polymorphic microsatellites (i.e. EST-SSRs) based on populations both from native (French coast) and invasive ranges (Canadian coast). The number of alleles, observed heterozygosity, and expected heterozygosity ranged from 2 to 12, 0.200 to 0.783, and 0.523 to 0.888 for the French population, and from 2 to 10, 0 to 0.524 and 0.043 to 0.827 for the Canadian population, respectively. We found significant population genetic differentiation between the native and invasive populations (pairwise F-ST = 0.1712). Moreover, principal coordinates analysis and Bayesian clustering test suggest long-distance dispersal between distant populations. When all loci were subjected for selection analyses, two loci (BS3244 and BS5339) were under selection based on the LOSITAN test. The results obtained in this study can help understand how ecological and evolutionary processes shape population genetic structure, and further how these processes contribute to invasion success. (C) 2016 Elsevier Ltd. All rights reserved.

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