Pseudooceanicola pacificus sp. nov., isolated from deep-sea sediment of the Pacific Ocean

• Published in: International Journal of Systematic and Evolutionary Microbiology
• Main Authors: Lyu, Lina, Lai, Qiliang, Li, Jianyang, Shao, Zongze, Yu, Zhiqiang
• Format: Report
• Language: English
• Published: MICROBIOLOGY SOC 2020
• Subjects: Microbiology; Pseudooceanicola pacificus sp. nov; deep-sea sediment; taxonomy; Pacific Ocean; MARINE ALPHAPROTEOBACTERIUM; EMENDED DESCRIPTION; GEN. NOV; FLAGELLATUS; RECLASSIFICATION; NITRATIREDUCENS; NANHAIENSIS; ANTARCTICUS; BATSENSIS; SEQUENCES; Pacific; Antarc*; antarcticus
• Online Access: http://ir.gig.ac.cn/handle/344008/60949; https://doi.org/10.1099/ijsem.0.004298

A Gram-strain-negative, rod-shaped, aerobic bacterium, designated 216_PA32_1(T), was isolated from deep-sea sediment of the Pacific Ocean. Cells of strain 216_PA32_1(T) were non-motile, oxidase-positive and catalase-negative. The strain could grow at temperatures of 10-45 degrees C (optimum, 32-35 degrees C), at pH 5.0-10.0 (optimum, 6.0-7.0) and at salinities of 0-10% (optimum, 2-8%). The principal fatty acid (10 %) was summed feature 8 (C-18:1 omega 6c/omega 7c). The sole respiratory quinone was Q10 (100 %). The polar lipids included phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, two unidentified phospholipids and five unidentified aminolipids. The G+C content of the chromosomal DNA was 66.3 mol%. According to the 16S rRNA gene similarity, strain 216_PA32_1(T) showed the highest sequence similarity to Pseudooceanicola nitratireducens JLT 1210(T) (97.3 %), followed by Pseudooceanicola nanhaiensis SS011B1-20(T) (97.1 %). Phylogenetic trees indicated that strain 216_PA32_1(T) clustered with strain P. nanhaiensis SS011B1-20(T). The average nucleotide identity and the DNA-DNA hybridization values between strain 216_PA32_1(T) and all species of the genus Pseudooceanicola were below 79.5 and 20.6%, respectively. A combination of the phylogenetic, phenotypic, chemotaxonomic and genomic evidence demonstrated that strain 216_PA32_1(T) represents a novel species of the genus Pseudooceanicola, for which the name Pseudooceanicola pacificus sp. nov. is proposed. The type strain is 216_PA32_1(T) (=MCCC 1A14128(T)=KCTC 72688(T)).