Pseudooceanicola pacificus sp. nov., isolated from deep-sea sediment of the Pacific Ocean

A Gram-strain-negative, rod-shaped, aerobic bacterium, designated 216_PA32_1(T), was isolated from deep-sea sediment of the Pacific Ocean. Cells of strain 216_PA32_1(T) were non-motile, oxidase-positive and catalase-negative. The strain could grow at temperatures of 10-45 degrees C (optimum, 32-35 d...

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Bibliographic Details
Published in:International Journal of Systematic and Evolutionary Microbiology
Main Authors: Lyu, Lina, Lai, Qiliang, Li, Jianyang, Shao, Zongze, Yu, Zhiqiang
Format: Report
Language:English
Published: MICROBIOLOGY SOC 2020
Subjects:
Microbiology
Pseudooceanicola pacificus sp. nov
deep-sea sediment
taxonomy
Pacific Ocean
MARINE ALPHAPROTEOBACTERIUM
EMENDED DESCRIPTION
GEN. NOV
FLAGELLATUS
RECLASSIFICATION
NITRATIREDUCENS
NANHAIENSIS
ANTARCTICUS
BATSENSIS
SEQUENCES
Pacific
Antarc*
antarcticus
Online Access:http://ir.gig.ac.cn/handle/344008/60949
https://doi.org/10.1099/ijsem.0.004298
Description
Summary:A Gram-strain-negative, rod-shaped, aerobic bacterium, designated 216_PA32_1(T), was isolated from deep-sea sediment of the Pacific Ocean. Cells of strain 216_PA32_1(T) were non-motile, oxidase-positive and catalase-negative. The strain could grow at temperatures of 10-45 degrees C (optimum, 32-35 degrees C), at pH 5.0-10.0 (optimum, 6.0-7.0) and at salinities of 0-10% (optimum, 2-8%). The principal fatty acid (10 %) was summed feature 8 (C-18:1 omega 6c/omega 7c). The sole respiratory quinone was Q10 (100 %). The polar lipids included phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, two unidentified phospholipids and five unidentified aminolipids. The G+C content of the chromosomal DNA was 66.3 mol%. According to the 16S rRNA gene similarity, strain 216_PA32_1(T) showed the highest sequence similarity to Pseudooceanicola nitratireducens JLT 1210(T) (97.3 %), followed by Pseudooceanicola nanhaiensis SS011B1-20(T) (97.1 %). Phylogenetic trees indicated that strain 216_PA32_1(T) clustered with strain P. nanhaiensis SS011B1-20(T). The average nucleotide identity and the DNA-DNA hybridization values between strain 216_PA32_1(T) and all species of the genus Pseudooceanicola were below 79.5 and 20.6%, respectively. A combination of the phylogenetic, phenotypic, chemotaxonomic and genomic evidence demonstrated that strain 216_PA32_1(T) represents a novel species of the genus Pseudooceanicola, for which the name Pseudooceanicola pacificus sp. nov. is proposed. The type strain is 216_PA32_1(T) (=MCCC 1A14128(T)=KCTC 72688(T)).

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