Proteomic analysis of serum in a population‐based cohort did not reveal a biomarker for Modic changes

IntroductionModic changes (MC) are bone marrow lesions of vertebral bones, which can be detected with magnetic resonance imaging (MRI) adjacent to degenerated intervertebral discs. Defined by their appearance on T1 and T2 weighted images, there are three interconvertible types: MC1, MC2, and MC3. Th...

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Bibliographic Details
Published in:JOR SPINE
Main Authors: Schulze, Friederike, Määttä, Juhani, Grad, Sybille, Heggli, Irina, Brunner, Florian, Farshad, Mazda, Distler, Oliver, Karppinen, Jaro, Lotz, Jeffrey, Dudli, Stefan
Format: Article in Journal/Newspaper
Language:unknown
Published: eScholarship, University of California 2024
Subjects:
Biomedical and Clinical Sciences
Clinical Sciences
Clinical Research
Biomedical Imaging
4.1 Discovery and preclinical testing of markers and technologies
4.2 Evaluation of markers and technologies
degenerative disc disease
low back pain
Modic changes
northern Finland birth cohort
proteomics
serum-biomarker
serum‐biomarker
Northern Finland
Online Access:https://escholarship.org/uc/item/3ht3j9d8
https://escholarship.org/content/qt3ht3j9d8/qt3ht3j9d8.pdf
https://doi.org/10.1002/jsp2.1337
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Summary:IntroductionModic changes (MC) are bone marrow lesions of vertebral bones, which can be detected with magnetic resonance imaging (MRI) adjacent to degenerated intervertebral discs. Defined by their appearance on T1 and T2 weighted images, there are three interconvertible types: MC1, MC2, and MC3. The inter-observer variability of the MRI diagnosis is high, therefore a diagnostic serum biomarker complementing the MRI to facilitate diagnosis and follow-up would be of great value.MethodsWe used a highly sensitive and reproducible proteomics approach: DIA/SWATH-MS to find serum biomarkers in a subset of the Northern Finland Birth Cohort 1966. Separately, we measured a panel of factors involved in inflammation and angiogenesis to confirm some potential biomarkers published before with an ELISA-based method called V-Plex.ResultsWe found neither an association between the serum concentrations of the proteins detected with DIA/SWATH-MS with the presence of MC, nor a correlation with the size of the MC lesions. We did not find any association between the factors measured with the V-Plex and the presence of MC or their size.ConclusionAltogether, our study suggests that a robust and generally usable biomarker to facilitate the diagnosis of MC cannot readily be found in serum.

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