Study of the adhesion phenomenon in the Pacific oyster larvae Crassostrea gigas at the pediveliger stage.

Oysters show a two-phase life cycle: pelagic larvae adhere before metamorphosis into benthic life. Larval adhesion occurs at the pediveliger stage by secretion of a bioadhesive produced by a specialized organ: the foot. The oyster Crassostrea gigas is an organism of economic and ecological importanc...

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Bibliographic Details
Main Author: Foulon, Valentin
Other Authors: Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Université de Bretagne occidentale - Brest, Claire Hellio, Fabienne Guérard, Pierre Boudry
Format: Doctoral or Postdoctoral Thesis
Language:French
Published: HAL CCSD 2018
Subjects:
Online Access:https://tel.archives-ouvertes.fr/tel-02130552
https://tel.archives-ouvertes.fr/tel-02130552v2/document
https://tel.archives-ouvertes.fr/tel-02130552v2/file/These-2018-SML-Biologie_marine-FOULON_Valentin.pdf
Description
Summary:Oysters show a two-phase life cycle: pelagic larvae adhere before metamorphosis into benthic life. Larval adhesion occurs at the pediveliger stage by secretion of a bioadhesive produced by a specialized organ: the foot. The oyster Crassostrea gigas is an organism of economic and ecological importance, and a model for study in marine biology, but the phenomenon of adhesion in the pediveliger larvae is poorly documented. A morphological description of the pediveliger larvae by histology and electron microscopy was performed to describe the glands responsible for the secretion of the adhesive.A predominantly proteinaceous composition of the adhesive was revealed by histochemistry and FTIR spectroscopy. An in silico analysis of available transcriptomic data from C. gigas was made to identify genes probably involved in adhesion. Two proteomic analyses, performed on whole larvae and on the secreted adhesive, characterizing proteins related to biosynthesis and adhesive structure. A collagen-like protein appears to be involved in the adhesive structure of C. gigas. This first approach to the study of the adhesion of C. gigas makes it possible to consider the biotechnological enhancement of the identified molecules. Despite their toxicity, synthetic adhesives dominate the world market. The development of biomimetic adhesives, based on marine bioadhesive strategies could be an alternative, and allowing furthermore bonding in wet condition. Les huîtres présentent un cycle de vie en deux phases : les larves pélagiques s’adhérent avant de se métamorphoser pour une vie benthique.L’adhésion larvaire se fait au stade pédivéligère par sécrétion d’un bioadhésif produit par un organe spécialisé : le pied. Bien que l’huître Crassostrea gigas soit un organisme d’importance économique et écologique, et un modèle d’étude en biologie marine, le phénomène d’adhésion chez la larve pédivéligère est peu documenté. Une étude morphologique des larves pédivéligères par histologie et microscopie électronique a été réalisée, afin de décrire ...