The marine intertidal zone shapes oyster and clam digestive bacterial microbiota

• Published in: FEMS Microbiology Ecology
• Main Authors: Offret, Clément, Paulino, Sauvann, Gauthier, Olivier, Château, Kevin, Bidault, Adeline, Corporeau, Charlotte, Miner, Philippe, Petton, Bruno, Pernet, Fabrice, Fabioux, Caroline, Paillard, Christine, Le Blay, Gwenaelle
• Other Authors: Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), This work was supported by ISblue project, Interdisciplinary graduate school for the blue planet (ANR-17-EURE-0015) and co-funded by a grant from the French government under the program "Investissements d'Avenir". The Region Bretagne SAD (2017, "Stratégie d'Attractivité Durable") contributed to this study through postdoctoral fellowship of Clément Offret. This work was also supportedby the HORIZON2020 project “Preventing and mitigating farmed bivalve disease—VIVALDI (grant number 678589)”, ANR-17-EURE-0015,ISBlue,Interdisciplinary Graduate School for the Blue planet(2017), European Project: 678589,H2020,H2020-SFS-2015-2,VIVALDI(2016)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2020
• Subjects: digestive gland; intertidal zone; clams; oysters; microbiota; metabarcoding; [SDV]Life Sciences [q-bio]; Crassostrea gigas
• Online Access: https://hal.archives-ouvertes.fr/hal-03083025; https://hal.archives-ouvertes.fr/hal-03083025/document; https://hal.archives-ouvertes.fr/hal-03083025/file/Offret%20et%20al.2019_Unedited.pdf; https://doi.org/10.1093/femsec/fiaa078

WOS:000574373300003 International audience Digestive microbiota provides a wide range of beneficial effects on host physiology and are therefore likely to play a key role in marine intertidal bivalve ability to acclimatize to the intertidal zone. This study investigated the effect of intertidal levels on the digestive bacterial microbiota of oysters Crassostrea gigas and clams Ruditapes philippinarum, two bivalves with different ecological niches. Based on the 16S rRNA region sequencing, digestive glands, seawater and sediments harbored specific bacterial communities, dominated by OTUs assigned to the Mycoplasmatales, Desulfobacterales and Rhodobacterales orders, respectively. Field implantation modified digestive bacterial microbiota of both bivalve species according to their intertidal position. Rhodospirillales and Legionellales abundances increased in oysters and clams from low intertidal level, respectively. After a 14-day depuration process, these effects were still observed especially for clams, while digestive bacterial microbiota of oysters were more subjected to short-term environmental changes. Nevertheless, 3.5 months stay on intertidal zone was enough to leave an environmental footprint on the digestive bacterial microbiota, suggesting the existence of autochthonous bivalve bacteria. When comparing clams from the three intertidal levels, 20% of the bacterial assemblage was shared among the levels and it was dominated by OTU affiliated to the Mycoplasmataceae and Spirochaetaceae families.